Literature DB >> 2584433

Detection of hepatitis B virus DNA in serum using synthetic non-radioactive oligonucleotides.

A Manzin1, P Pauri, P Bagnarelli, F Brecciaroli, P E Varaldo, S Colloca, M Clementi.   

Abstract

A rapid and simplified technique for detecting hepatitis B virus (HBV) DNA by spot hybridisation in the sera of patients with different clinical forms of HBV infection was investigated using enzyme conjugated synthetic oligodeoxyribonucleotides as probes. These are able to hybridize to the S and C regions of the HBV L(-) DNA strand. When compared with a complete 32P-labelled HBV DNA probe, the synthetic oligonucleotides provided a sensitive and quick method for the routine survey of HBV infection. Moreover, the DNA extraction procedure used allowed the spot hybridisation technique to be applied and read easily and the results obtained within a few hours. It is concluded that synthetic cold probes can be used in hybridisation assays HBV DNA detection as part of current clinical laboratory procedures.

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Year:  1989        PMID： 2584433      PMCID： PMC501983          DOI： 10.1136/jcp.42.11.1206

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  21 in total

1.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

2.  Detection of HBV infectivity by spot hybridization in HBeAg-negative chronic carriers: HBV DNA in sera from asymptomatic and symptomatic subjects.

Authors:  G Carloni; S Colloca; C Delfini; A Manzin; M Clementi; F Galibert
Journal:  J Med Virol       Date:  1987-01       Impact factor: 2.327

3.  The importance of hepatitis B viral DNA in serum and liver.

Authors:  F Bonino
Journal:  J Hepatol       Date:  1986       Impact factor: 25.083

4.  Detection of hepatitis B virus DNA in serum by a simple spot hybridization technique: comparison with results for other viral markers.

Authors:  J Scotto; M Hadchouel; C Hery; J Yvart; P Tiollais; C Brechot
Journal:  Hepatology       Date:  1983 May-Jun       Impact factor: 17.425

5.  Non-radioactive hepatitis B virus DNA probe for detection of HBV-DNA in serum.

Authors:  D Larzul; V Thiers; A M Courouce; C Bréchot; J L Guesdon
Journal:  J Hepatol       Date:  1987-10       Impact factor: 25.083

6.  State of hepatitis B virus DNA in leucocytes of hepatitis B patients.

Authors:  J R Gu; Y C Chen; H Q Jiang; Y L Zhang; S M Wu; W L Jiang; J Jian
Journal:  J Med Virol       Date:  1985-09       Impact factor: 2.327

7.  An oligonucleotide probe for the detection of hepatitis B virus DNA in serum.

Authors:  H J Lin; P C Wu; C L Lai
Journal:  J Virol Methods       Date:  1987-02       Impact factor: 2.014

8.  Incidence of hepatitis B virus DNA and DNA-polymerase in sera of Italian asymptomatic carriers with the serological markers of HBV.

Authors:  G Carloni; C Delfini; S Colloca; E Alfani; G Taliani; C De Bac
Journal:  Arch Virol       Date:  1986       Impact factor: 2.574

Review 9.  The hepatitis B virus.

Authors:  P Tiollais; C Pourcel; A Dejean
Journal:  Nature       Date:  1985 Oct 10-16       Impact factor: 49.962

10.  Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes.

Authors:  D J Brigati; D Myerson; J J Leary; B Spalholz; S Z Travis; C K Fong; G D Hsiung; D C Ward
Journal:  Virology       Date:  1983-04-15       Impact factor: 3.616
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  1 in total

1.  Evaluation of enzyme immunoassay for hepatitis B virus DNA based on anti-double-stranded DNA.

Authors:  F Garcia; F Garcia; M C Bernal; A Leyva; G Piedrola; M C Maroto
Journal:  J Clin Microbiol       Date:  1995-02       Impact factor: 5.948
  1 in total

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