| Literature DB >> 25843841 |
V N Davydova1, A V Volod'ko2, E V Sokolova2, E A Chusovitin3, S A Balagan3, V I Gorbach2, N G Galkin4, I M Yermak2, T F Solov'eva2.
Abstract
The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy. The alteration of ζ-potential of E-LPS-Ch complexes from negative to positive values depending on Ch content was detected. The Y-LPS-Ch complexes had similar positive ζ-potentials regardless of Ch content. The transformation of the supramolecular structure of E-LPS after binding with to Ch was revealed. Screening of E-LPS and Y-LPS particles by Ch in the complexes with high polycation was detected. The ability of LPS-Ch complex to induce biosynthesis of TNF-α and reactive oxygen species in stimulated human mononuclear cells was studied. A significant decrease in activity complexes compared to that of the initial LPS was observed only for E-LPS-Ch complexes.Entities:
Keywords: Chitosan; Chitosan (PubChem CID: 71853); LPS–chitosan complexes; Lipopolysaccharide (LPS); Lipopolysaccharide (PubChem CID: 11970143); Supramolecular structure; ζ-Potential
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Year: 2015 PMID： 25843841 DOI： 10.1016/j.carbpol.2015.01.028
Source DB: PubMed Journal: Carbohydr Polym ISSN： 0144-8617 Impact factor: 9.381