| Literature DB >> 25841748 |
Yi Tang1, Huaguang Lu2, Aswathy Sebastian3, Yin-Ting Yeh4, Craig A Praul5, Istvan U Albert3, Si-Yang Zheng4.
Abstract
The genome of a turkey arthritis reovirus (TARV) field strain (Reo/PA/Turkey/22342/13), isolated from a turkey flock in Pennsylvania (PA) in 2013, has been sequenced using Next-Generation Sequencing (NGS) on the Illumina MiSeq platform. The genome of the PA TARV field strain was 23,496bp in length with 10 dsRNA segments encoding 12 viral proteins. The lengths of the genomic segments ranged from 1192bp (S4) to 3959bp (L1). The 5' and 3' conserved terminal sequences of the PA TARV field strain were similar to the two Minnesota (MN) TARVs (MN9 and MN10) published recently and avian orthoreovirus (ARV) reference strains. Phylogenetic analysis of the nucleotide sequences of all 10 genome segments revealed that there was a low to significant nucleotide sequence divergence between the PA TARV field strain and reference TARV and ARV strains. Analysis of the PA TARV sequence indicates that this PA TARV field strain is a unique strain and is different from the TARV MN9 or MN10 in M2 segment genes and ARV S1133 vaccine strain.Entities:
Keywords: Genome segments; Genotyping; Next-Generation Sequencing; Turkey arthritis reovirus
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Year: 2015 PMID： 25841748 PMCID： PMC4649943 DOI： 10.1016/j.meegid.2015.03.029
Source DB: PubMed Journal: Infect Genet Evol ISSN： 1567-1348 Impact factor: 3.342