Literature DB >> 25840367

Study of the immunogenicity of hepatitis B surface antigen synthesized in transgenic potato plants with increased biosafety.

Elena B Rukavtsova1, Natalya V Rudenko2, Elena N Puchko2, Natalya S Zakharchenko2, Yaroslav I Buryanov2.   

Abstract

Oral immunogenicity of the hepatitis B surface antigen (HBsAg) synthesized in the tubers of marker-free potato plants has been demonstrated. Experiments were performed in the two groups of outbred NMRI mice. At the beginning of investigations, the mice of experimental group were fed the tubers of transgenic potato synthesizing the HBsAg three times. The mice of control group were fed nontransgenic potato. Intraperitoneal injection of the commercial vaccine against hepatitis B (0.5μg/mouse) was made on day 71 of the experiment. Enzyme-linked immunoassay (ELISA) of the serum of immunized animals showed an increase in the level of HBsAg antibodies significantly above the protective value, which was maintained for 1 year after the immunization. In 1 year, the experimental group of mice underwent additional oral immunization with HBsAg-containing potato tubers. As a result, the level of antibodies against the HBsAg increased and remained at a high protective level for several months. The findings show the possibility of using transgenic plants as a substance for obtaining a safe edible vaccine against hepatitis B.
Copyright © 2015 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  ELISA; Hepatitis B surface antigen; Immunogenicity; Pre-clinical trials; Transgenic marker-free potato plants

Mesh:

Substances:

Year:  2015        PMID: 25840367     DOI: 10.1016/j.jbiotec.2015.03.019

Source DB:  PubMed          Journal:  J Biotechnol        ISSN: 0168-1656            Impact factor:   3.307


  6 in total

Review 1.  Edible plants for oral delivery of biopharmaceuticals.

Authors:  Matilde Merlin; Mario Pezzotti; Linda Avesani
Journal:  Br J Clin Pharmacol       Date:  2016-05-09       Impact factor: 4.335

Review 2.  Green Revolution to Gene Revolution: Technological Advances in Agriculture to Feed the World.

Authors:  Mohd Fadhli Hamdan; Siti Nurfadhlina Mohd Noor; Nazrin Abd-Aziz; Teen-Lee Pua; Boon Chin Tan
Journal:  Plants (Basel)       Date:  2022-05-12

Review 3.  Using transgenic plants and modified plant viruses for the development of treatments for human diseases.

Authors:  Hwei-San Loh; Brian J Green; Vidadi Yusibov
Journal:  Curr Opin Virol       Date:  2017-08-08       Impact factor: 7.090

4.  Development of a Transgenic Flammulina velutipes Oral Vaccine for Hepatitis B.

Authors:  Li-Hsin Huang; Hao-Yeh Lin; Ying-Tzu Lyu; Chiau-Ling Gung; Ching-Tsan Huang
Journal:  Food Technol Biotechnol       Date:  2019-03       Impact factor: 3.918

Review 5.  Need of cost-effective vaccines in developing countries: What plant biotechnology can offer?

Authors:  Mohammad Tahir Waheed; Muhammad Sameeullah; Faheem Ahmed Khan; Tahira Syed; Manzoor Ilahi; Johanna Gottschamel; Andreas Günter Lössl
Journal:  Springerplus       Date:  2016-01-22

Review 6.  The Last Ten Years of Advancements in Plant-Derived Recombinant Vaccines against Hepatitis B.

Authors:  Young Hee Joung; Se Hee Park; Ki-Beom Moon; Jae-Heung Jeon; Hye-Sun Cho; Hyun-Soon Kim
Journal:  Int J Mol Sci       Date:  2016-10-13       Impact factor: 5.923

  6 in total

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