| Literature DB >> 25839413 |
Sabine Hauptstein1, Felix Prüfert1, Andreas Bernkop-Schnürch2.
Abstract
It was the aim of this study to investigate a novel strategy for oral gene delivery utilizing a self-nanoemulsifying drug delivery system (SNEDDS). After hydrophobic ion pairing a plasmid was incorporated into SNEDDS. The mean droplet size of resulting nanoemulsions was determined to be between 45.8 and 47.5 nm. A concentration dependent cytotoxicity of the formulations was found on HEK-293 cells via MTT assay. Degradation studies via DNase I showed that incorporation into SNEDDS led to significantly, up to 8-fold prolonged resistant time against enzymatic digestion compared to naked pDNA and pDNA-lipid complexes. Transfection studies carried out revealed a significantly improved transfection compared to naked pDNA. Further, no decrease in transfection efficiency compared to transfection using Lipofectin(®) transfection reagent was observed.Entities:
Keywords: 1-Hexadecylpyridin-1-ium;chloride (PubChem CID: 31239); 2,3-Bis[(Z)-octadec-9-enoxy]propyl-trimethylazanium;chloride (PubChem CID: 6438350); Cremophor-EL (PubChem CID 5849927); Dodecyl(trimethyl)azanium;bromide (PubChem CID: 14249); Gene delivery; Hexadecyl(trimethyl)azanium;bromide (PubChem CID: 5974); Hydrophobic ion pairing; Nonviral vector; SNEDDS; [3-[2-Aminoethoxy(hydroxy)phosphoryl]oxy-2-[(Z)-octadec-9-enoyl]oxypropyl] (Z)-octadec-9-enoate (PubChem CID: 6437392)
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Year: 2015 PMID： 25839413 DOI： 10.1016/j.ijpharm.2015.03.064
Source DB: PubMed Journal: Int J Pharm ISSN： 0378-5173 Impact factor: 5.875