Miguel L O'Ryan1, Yalda Lucero2, Marcela Rabello2, Nora Mamani1, Ana María Salinas3, Alfredo Peña4, Juan Pablo Torres-Torreti2, Asunción Mejías5, Octavio Ramilo5, Nicolas Suarez5, Henry E Reynolds6, Andrea Orellana1, Anne J Lagomarcino1. 1. Microbiology and Mycology Program, Institute of Biomedical Sciences. 2. Luis Calvo Mackenna Hospital, Department of Pediatrics and Pediatric Surgery (Eastern Campus), Faculty of Medicine, Universidad de Chile. 3. School of Medical Technology, Faculty of Health, Universidad Santo Tomás. 4. Pediatric Service, Sótero del Río Hospital, Santiago, Chile. 5. Center for Vaccines and Immunity, The Research Institute at Nationwide Children's Hospital, The Ohio State University School of Medicine, Columbus. 6. Physiopathology Program Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Santiago.
Abstract
BACKGROUND: Helicobacter pylori, the main cause of peptic ulcer disease and gastric cancer in adult populations, is generally acquired during the first years of life. Infection can be persistent or transient and bacterial and host factors determining persistence are largely unknown and may prove relevant for future disease. METHODS: Two cohorts of healthy Chilean infants (313 total) were evaluated every 3 months for 18-57 months to determine pathogen- and host-factors associated with persistent and transient infection. RESULTS: One-third had at least one positive stool ELISA by age 3, with 20% overall persistence. Persistent infections were acquired at an earlier age, associated with more household members, decreased duration of breastfeeding, and nonsecretor status compared to transient infections. The cagA positive strains were more common in persistent stools, and nearly 60% of fully characterized persistent stool samples amplified cagA/vacAs1m1. Persistent children were more likely to elicit a serologic immune response, and both infection groups had differential gene expression profiles, including genes associated with cancer suppression when compared to healthy controls. CONCLUSIONS: These results indicate that persistent H. pylori infections acquired early in life are associated with specific host and/or strain profiles possibly associated with future disease occurrence.
BACKGROUND:Helicobacter pylori, the main cause of peptic ulcer disease and gastric cancer in adult populations, is generally acquired during the first years of life. Infection can be persistent or transient and bacterial and host factors determining persistence are largely unknown and may prove relevant for future disease. METHODS: Two cohorts of healthy Chilean infants (313 total) were evaluated every 3 months for 18-57 months to determine pathogen- and host-factors associated with persistent and transient infection. RESULTS: One-third had at least one positive stool ELISA by age 3, with 20% overall persistence. Persistent infections were acquired at an earlier age, associated with more household members, decreased duration of breastfeeding, and nonsecretor status compared to transient infections. The cagA positive strains were more common in persistent stools, and nearly 60% of fully characterized persistent stool samples amplified cagA/vacAs1m1. Persistent children were more likely to elicit a serologic immune response, and both infection groups had differential gene expression profiles, including genes associated with cancer suppression when compared to healthy controls. CONCLUSIONS: These results indicate that persistent H. pyloriinfections acquired early in life are associated with specific host and/or strain profiles possibly associated with future disease occurrence.
Authors: Sabine Kienesberger; Guillermo I Perez-Perez; Asalia Z Olivares; Pradip Bardhan; Shafiqul A Sarker; Kh Zahid Hasan; R Bradley Sack; Martin J Blaser Journal: Gut Microbes Date: 2018-03-01
Authors: Andrea Orellana-Manzano; Miguel G O'Ryan; Anne J Lagomarcino; Sergio George; Mindy S Muñoz; Nora Mamani; Carolina A Serrano; Paul R Harris; Octavio Ramilo; Asunción Mejías; Juan P Torres; Yalda Lucero; Andrew F G Quest Journal: Front Cell Infect Microbiol Date: 2016-10-10 Impact factor: 5.293
Authors: Edity Namyalo; Luke Nyakarahuka; Matthias Afayoa; Joel Baziira; Andrew Tamale; G Collins Atuhaire; Joseph M Kungu Journal: J Trop Med Date: 2021-11-08