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Literature DB >> 25837389

In vivo investigation of cilia structure and function using Xenopus.

Abstract

Cilia are key organelles in development and homeostasis. The ever-expanding complement of cilia associated proteins necessitates rapid and tractable models for in vivo functional investigation. Xenopus laevis provides an attractive model for such studies, having multiple ciliated populations, including primary and multiciliated tissues. The rapid external development of Xenopus and the large cells make it an especially excellent platform for imaging studies. Here we present embryological and cell biological methods for the investigation of cilia structure and function in X. laevis, with a focus on quantitative live and fixed imaging.

Entities: Chemical Disease Gene Species

Keywords: Basal bodies; Cilia; Confocal microscopy; Intraflagellar transport; Multiciliated cells; Quantitative imaging; Xenopus

Mesh：

Substances：

Year: 2015 PMID： 25837389 PMCID： PMC4433029 DOI： 10.1016/bs.mcb.2015.01.018

Source DB: PubMed Journal: Methods Cell Biol ISSN： 0091-679X Impact factor: 1.441

58 in total

Review 1. The reproductive significance of human Fallopian tube cilia.

Authors: R A Lyons; E Saridogan; O Djahanbakhch
Journal: Hum Reprod Update Date: 2006-03-24 Impact factor: 15.610

2. New neurons follow the flow of cerebrospinal fluid in the adult brain.

Authors: Kazunobu Sawamoto; Hynek Wichterle; Oscar Gonzalez-Perez; Jeremy A Cholfin; Masayuki Yamada; Nathalie Spassky; Noel S Murcia; Jose Manuel Garcia-Verdugo; Oscar Marin; John L R Rubenstein; Marc Tessier-Lavigne; Hideyuki Okano; Arturo Alvarez-Buylla
Journal: Science Date: 2006-01-12 Impact factor: 47.728

Review 3. Controlling morpholino experiments: don't stop making antisense.

Authors: Judith S Eisen; James C Smith
Journal: Development Date: 2008-04-09 Impact factor: 6.868

4. EB1 and EB3 promote cilia biogenesis by several centrosome-related mechanisms.

Authors: Jacob M Schrøder; Jesper Larsen; Yulia Komarova; Anna Akhmanova; Rikke I Thorsteinsson; Ilya Grigoriev; Robert Manguso; Søren T Christensen; Stine F Pedersen; Stefan Geimer; Lotte B Pedersen
Journal: J Cell Sci Date: 2011-08-01 Impact factor: 5.285

5. Investigating morphogenesis in Xenopus embryos: imaging strategies, processing, and analysis.

Authors: Hye Young Kim; Lance A Davidson
Journal: Cold Spring Harb Protoc Date: 2013-04-01

Review 6. Understanding ciliated epithelia: the power of Xenopus.

Authors: M E Werner; B J Mitchell
Journal: Genesis Date: 2011-12-27 Impact factor: 2.487

7. The PCP pathway instructs the planar orientation of ciliated cells in the Xenopus larval skin.

Authors: Brian Mitchell; Jennifer L Stubbs; Fawn Huisman; Peter Taborek; Clare Yu; Chris Kintner
Journal: Curr Biol Date: 2009-05-07 Impact factor: 10.834

8. ANKS6 is a central component of a nephronophthisis module linking NEK8 to INVS and NPHP3.

Authors: Sylvia Hoff; Jan Halbritter; Daniel Epting; Valeska Frank; Thanh-Minh T Nguyen; Jeroen van Reeuwijk; Christopher Boehlke; Christoph Schell; Takayuki Yasunaga; Martin Helmstädter; Miriam Mergen; Emilie Filhol; Karsten Boldt; Nicola Horn; Marius Ueffing; Edgar A Otto; Tobias Eisenberger; Mariet W Elting; Joanna A E van Wijk; Detlef Bockenhauer; Neil J Sebire; Søren Rittig; Mogens Vyberg; Troels Ring; Martin Pohl; Lars Pape; Thomas J Neuhaus; Neveen A Soliman Elshakhs; Sarah J Koon; Peter C Harris; Florian Grahammer; Tobias B Huber; E Wolfgang Kuehn; Albrecht Kramer-Zucker; Hanno J Bolz; Ronald Roepman; Sophie Saunier; Gerd Walz; Friedhelm Hildebrandt; Carsten Bergmann; Soeren S Lienkamp
Journal: Nat Genet Date: 2013-06-23 Impact factor: 38.330

9. Myb promotes centriole amplification and later steps of the multiciliogenesis program.

Authors: Fraser E Tan; Eszter K Vladar; Lina Ma; Luis C Fuentealba; Ramona Hoh; F Hernán Espinoza; Jeffrey D Axelrod; Arturo Alvarez-Buylla; Tim Stearns; Chris Kintner; Mark A Krasnow
Journal: Development Date: 2013-09-18 Impact factor: 6.868

10. The Small GTPase Rsg1 is important for the cytoplasmic localization and axonemal dynamics of intraflagellar transport proteins.

Authors: Eric R Brooks; John B Wallingford
Journal: Cilia Date: 2013-10-07

9 in total

Review 1. What we can learn from a tadpole about ciliopathies and airway diseases: Using systems biology in Xenopus to study cilia and mucociliary epithelia.

Authors: Peter Walentek; Ian K Quigley
Journal: Genesis Date: 2017-01 Impact factor: 2.487

9. Protein turnover dynamics suggest a diffusion-to-capture mechanism for peri-basal body recruitment and retention of intraflagellar transport proteins.

Authors: Jaime V K Hibbard; Neftali Vazquez; Rohit Satija; John B Wallingford
Journal: Mol Biol Cell Date: 2021-04-07 Impact factor: 4.138

9 in total

In vivo investigation of cilia structure and function using Xenopus.

Review 1. The reproductive significance of human Fallopian tube cilia.

2. New neurons follow the flow of cerebrospinal fluid in the adult brain.

Review 3. Controlling morpholino experiments: don't stop making antisense.

4. EB1 and EB3 promote cilia biogenesis by several centrosome-related mechanisms.

5. Investigating morphogenesis in Xenopus embryos: imaging strategies, processing, and analysis.

Review 6. Understanding ciliated epithelia: the power of Xenopus.

7. The PCP pathway instructs the planar orientation of ciliated cells in the Xenopus larval skin.

8. ANKS6 is a central component of a nephronophthisis module linking NEK8 to INVS and NPHP3.

9. Myb promotes centriole amplification and later steps of the multiciliogenesis program.

10. The Small GTPase Rsg1 is important for the cytoplasmic localization and axonemal dynamics of intraflagellar transport proteins.

Review 1. What we can learn from a tadpole about ciliopathies and airway diseases: Using systems biology in Xenopus to study cilia and mucociliary epithelia.

2. Identifying domains of EFHC1 involved in ciliary localization, ciliogenesis, and the regulation of Wnt signaling.

3. Kif9 is an active kinesin motor required for ciliary beating and proximodistal patterning of motile axonemes.

4. Septin-dependent remodeling of cortical microtubule drives cell reshaping during epithelial wound healing.

5. WDR5 Stabilizes Actin Architecture to Promote Multiciliated Cell Formation.

6. Whole-Mount RNA In Situ Hybridization and Immunofluorescence of Xenopus Embryos and Tadpoles.

Review 7. PCD Genes-From Patients to Model Organisms and Back to Humans.

8. Centrin diversity and basal body patterning across evolution: new insights from Paramecium.

9. Protein turnover dynamics suggest a diffusion-to-capture mechanism for peri-basal body recruitment and retention of intraflagellar transport proteins.