Literature DB >> 25837312

Proteomics characterization of exosome cargo.

Kevin L Schey1, J Matthew Luther2, Kristie L Rose3.   

Abstract

Characterization of exosomal cargo is of significant interest because this cargo can provide clues to exosome biogenesis, targeting, and cellular effects and may be a source of biomarkers for disease diagnosis, prognosis and response to treatment. With recent improvements in proteomics technologies, both qualitative and quantitative characterization of exosomal proteins is possible. Here we provide a brief review of exosome proteomics studies and provide detailed protocols for global qualitative, global quantitative, and targeted quantitative analysis of exosomal proteins. In addition, we provide an example application of a standard global quantitative analysis followed by validation via a targeted quantitative analysis of urine exosome samples from human patients. Advantages and limitations of each method are discussed as well as future directions for exosome proteomics analysis.
Copyright © 2015 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Mass spectrometry; Post-translational modifications; Proteomics

Mesh：

Substances：

Year:  2015        PMID： 25837312      PMCID： PMC4591097          DOI： 10.1016/j.ymeth.2015.03.018

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  48 in total

1.  Identification and proteomic profiling of exosomes in human urine.

Authors:  Trairak Pisitkun; Rong-Fong Shen; Mark A Knepper
Journal:  Proc Natl Acad Sci U S A       Date:  2004-08-23       Impact factor: 11.205

2.  The absolute quantification strategy: a general procedure for the quantification of proteins and post-translational modifications.

Authors:  Donald S Kirkpatrick; Scott A Gerber; Steven P Gygi
Journal:  Methods       Date:  2005-01-12       Impact factor: 3.608

Review 3.  Exosomes: proteomic insights and diagnostic potential.

Authors:  Richard J Simpson; Justin We Lim; Robert L Moritz; Suresh Mathivanan
Journal:  Expert Rev Proteomics       Date:  2009-06       Impact factor: 3.940

4.  Practical implementation of 2D HPLC scheme with accurate peptide retention prediction in both dimensions for high-throughput bottom-up proteomics.

Authors:  Ravi C Dwivedi; Vic Spicer; Michael Harder; Mihaela Antonovici; Werner Ens; Kenneth G Standing; John A Wilkins; Oleg V Krokhin
Journal:  Anal Chem       Date:  2008-08-08       Impact factor: 6.986

5.  Vesicle formation during reticulocyte maturation. Association of plasma membrane activities with released vesicles (exosomes).

Authors:  R M Johnstone; M Adam; J R Hammond; L Orr; C Turbide
Journal:  J Biol Chem       Date:  1987-07-05       Impact factor: 5.157

Review 6.  Advances in membranous vesicle and exosome proteomics improving biological understanding and biomarker discovery.

Authors:  Francesca Raimondo; Lavinia Morosi; Clizia Chinello; Fulvio Magni; Marina Pitto
Journal:  Proteomics       Date:  2011-01-17       Impact factor: 3.984

Review 7.  Contribution of proteomics to understanding the role of tumor-derived exosomes in cancer progression: state of the art and new perspectives.

Authors:  Simona Fontana; Fontana Simona; Laura Saieva; Saieva Laura; Simona Taverna; Taverna Simona; Riccardo Alessandro; Alessandro Riccardo
Journal:  Proteomics       Date:  2013-03-22       Impact factor: 3.984

8.  Using iRT, a normalized retention time for more targeted measurement of peptides.

Authors:  Claudia Escher; Lukas Reiter; Brendan MacLean; Reto Ossola; Franz Herzog; John Chilton; Michael J MacCoss; Oliver Rinner
Journal:  Proteomics       Date:  2012-04       Impact factor: 3.984

Review 9.  Post-translational modifications of exosomal proteins.

Authors:  Olga Moreno-Gonzalo; Carolina Villarroya-Beltri; Francisco Sánchez-Madrid
Journal:  Front Immunol       Date:  2014-08-11       Impact factor: 7.561

10.  Detection of tumor cell-specific mRNA and protein in exosome-like microvesicles from blood and saliva.

Authors:  Jieping Yang; Fang Wei; Christopher Schafer; David T W Wong
Journal:  PLoS One       Date:  2014-11-14       Impact factor: 3.240
View more
  52 in total

1.  Arthropod EVs mediate dengue virus transmission through interaction with a tetraspanin domain containing glycoprotein Tsp29Fb.

Authors:  Ashish Vora; Wenshuo Zhou; Berlin Londono-Renteria; Michael Woodson; Michael B Sherman; Tonya M Colpitts; Girish Neelakanta; Hameeda Sultana
Journal:  Proc Natl Acad Sci U S A       Date:  2018-06-26       Impact factor: 11.205

2.  Alcohol Increases Exosome Release from Microglia to Promote Complement C1q-Induced Cellular Death of Proopiomelanocortin Neurons in the Hypothalamus in a Rat Model of Fetal Alcohol Spectrum Disorders.

Authors:  Sayani Mukherjee; Miguel A Cabrera; Nadka I Boyadjieva; Gregory Berger; Bénédicte Rousseau; Dipak K Sarkar
Journal:  J Neurosci       Date:  2020-09-04       Impact factor: 6.167

3.  Extracellular histones are the ligands for the uptake of exosomes and hydroxyapatite-nanoparticles by tumor cells via syndecan-4.

Authors:  Josiah Ochieng; Gladys Nangami; Amos Sakwe; Tanu Rana; Shalonda Ingram; Jeffrey S Goodwin; Cierra Moye; Philip Lammers; Samuel E Adunyah
Journal:  FEBS Lett       Date:  2018-09-20       Impact factor: 4.124

4.  One-Pot Exosome Proteomics Enabled by a Photocleavable Surfactant.

Authors:  Kevin M Buck; David S Roberts; Timothy J Aballo; David R Inman; Song Jin; Suzanne Ponik; Kyle A Brown; Ying Ge
Journal:  Anal Chem       Date:  2022-05-11       Impact factor: 8.008

Review 5.  The updated role of exosomal proteins in the diagnosis, prognosis, and treatment of cancer.

Authors:  Xinyi Wang; Jing Huang; Wenjie Chen; Genpeng Li; Zhihui Li; Jianyong Lei
Journal:  Exp Mol Med       Date:  2022-09-22       Impact factor: 12.153

6.  Neuropilin-1 is present on Foxp3+ T regulatory cell-derived small extracellular vesicles and mediates immunity against skin transplantation.

Authors:  Mauricio Campos-Mora; Javiera De Solminihac; Carolina Rojas; Cristina Padilla; Mónica Kurte; Rodrigo Pacheco; Thilo Kaehne; Úrsula Wyneken; Karina Pino-Lagos
Journal:  J Extracell Vesicles       Date:  2022-06

Review 7.  Pancreatic cancer stem cell markers and exosomes - the incentive push.

Authors:  Sarah Heiler; Zhe Wang; Margot Zöller
Journal:  World J Gastroenterol       Date:  2016-07-14       Impact factor: 5.742

Review 8.  Unraveling the mechanisms that specify molecules for secretion in extracellular vesicles.

Authors:  Andrew M Leidal; Jayanta Debnath
Journal:  Methods       Date:  2020-01-21       Impact factor: 3.608

9.  Insights into the Proteome of Gastrointestinal Stromal Tumors-Derived Exosomes Reveals New Potential Diagnostic Biomarkers.

Authors:  Safinur Atay; Daniel W Wilkey; Mohammed Milhem; Michael Merchant; Andrew K Godwin
Journal:  Mol Cell Proteomics       Date:  2017-12-14       Impact factor: 5.911

Review 10.  Post-translational add-ons mark the path in exosomal protein sorting.

Authors:  Olga Moreno-Gonzalo; Irene Fernandez-Delgado; Francisco Sanchez-Madrid
Journal:  Cell Mol Life Sci       Date:  2017-10-27       Impact factor: 9.261
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.