P-F Chang1, Y-C Lin1, K Liu2, S-J Yeh1, Y-H Ni3. 1. 1] Department of Pediatrics, Far Eastern Memorial Hospital, Pan-Chiao, New Taipei, Taiwan [2] Department of Healthcare Administration, Oriental Institute of Technology, Pan-Chiao, New Taipei, Taiwan. 2. Department of Pediatrics, Far Eastern Memorial Hospital, Pan-Chiao, New Taipei, Taiwan. 3. Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan.
Abstract
BACKGROUND AND OBJECTIVES: Oxidative stress and the insulin-resistant state are thought to be key components in the pathogenesis of pediatric nonalcoholic fatty liver disease (NAFLD). Heme oxygenase (HO) is important in the defense against oxidative stress. This study aimed to assess the association of HO-1 gene promoter polymorphism and insulin resistance with NAFLD among obese children. METHODS: A total of 101 obese children aged 6-17 years were recruited. Anthropometric, serum biochemical variables and biomarkers for glucose and insulin metabolism were measured. We screened the allelic frequencies of (GT)n repeats in the HO-1 gene promoter among these obese children. NAFLD was determined through liver ultrasonography. Because the distribution of numbers of (GT)n repeats was bimodal, we divided the alleles into two classes: class S included shorter (27) repeats, and class L included longer (⩾27) repeats. We assessed the effects of the length of (GT)n repeats in HO-1 gene promoter on pediatric NAFLD. RESULTS: Of the 101 obese subjects, 27 (26.7%) had NAFLD. The alanine aminotransferase level was higher in patients carrying L alleles (L/L and L/S) than patients with S alleles (S/S) (46.2±49.3 IU|(-1) versus 30.2±20.1 IU|(-1); P=0.027). The significant risk factors for pediatric NAFLD were patients carrying L alleles (L/L and L/S) (odds ratio (OR)=18.84; 95% confidence interval (CI): 1.45-245.22; P=0.025), homeostasis model assessment of insulin resistance (OR=1.40; 95% CI: 1.07-1.83; P=0.014) and age (OR=1.24; 95% CI: 1.03-1.50; P=0.025). CONCLUSION: In this hospital-based study, the obese children with longer GT repeats in the HO-1 gene promoter and insulin resistance were susceptible to NAFLD.
BACKGROUND AND OBJECTIVES: Oxidative stress and the insulin-resistant state are thought to be key components in the pathogenesis of pediatric nonalcoholic fatty liver disease (NAFLD). Heme oxygenase (HO) is important in the defense against oxidative stress. This study aimed to assess the association of HO-1 gene promoter polymorphism and insulin resistance with NAFLD among obesechildren. METHODS: A total of 101 obesechildren aged 6-17 years were recruited. Anthropometric, serum biochemical variables and biomarkers for glucose and insulin metabolism were measured. We screened the allelic frequencies of (GT)n repeats in the HO-1 gene promoter among these obesechildren. NAFLD was determined through liver ultrasonography. Because the distribution of numbers of (GT)n repeats was bimodal, we divided the alleles into two classes: class S included shorter (27) repeats, and class L included longer (⩾27) repeats. We assessed the effects of the length of (GT)n repeats in HO-1 gene promoter on pediatric NAFLD. RESULTS: Of the 101 obese subjects, 27 (26.7%) had NAFLD. The alanine aminotransferase level was higher in patients carrying L alleles (L/L and L/S) than patients with S alleles (S/S) (46.2±49.3 IU|(-1) versus 30.2±20.1 IU|(-1); P=0.027). The significant risk factors for pediatric NAFLD were patients carrying L alleles (L/L and L/S) (odds ratio (OR)=18.84; 95% confidence interval (CI): 1.45-245.22; P=0.025), homeostasis model assessment of insulin resistance (OR=1.40; 95% CI: 1.07-1.83; P=0.014) and age (OR=1.24; 95% CI: 1.03-1.50; P=0.025). CONCLUSION: In this hospital-based study, the obesechildren with longer GT repeats in the HO-1 gene promoter and insulin resistance were susceptible to NAFLD.
Authors: I Janssen; P T Katzmarzyk; W F Boyce; C Vereecken; C Mulvihill; C Roberts; C Currie; W Pickett Journal: Obes Rev Date: 2005-05 Impact factor: 9.213
Authors: Joao Seda Neto; Atsunori Nakao; Kei Kimizuka; Anna Jeanine Romanosky; Donna B Stolz; Takashi Uchiyama; Michael A Nalesnik; Leo E Otterbein; Noriko Murase Journal: Am J Physiol Renal Physiol Date: 2004-08-03
Authors: Jun Yu; Eagle S H Chu; Ruizhi Wang; Shiyan Wang; Chung W Wu; Vincent W S Wong; Henry L Y Chan; Geofferey C Farrell; Joseph J Y Sung Journal: Gastroenterology Date: 2009-10-07 Impact factor: 22.682
Authors: Sabrina Xin Zi Quek; Eunice Xiang-Xuan Tan; Yi Ping Ren; Mark Muthiah; Evelyn Xiu Ling Loo; Elizabeth Huiwen Tham; Kewin Tien Ho Siah Journal: World J Hepatol Date: 2022-06-27
Authors: Jaromír Petrtýl; Karel Dvořák; Jan Stříteský; Martin Leníček; Alena Jirásková; Václav Šmíd; Martin Haluzík; Radan Brůha; Libor Vítek Journal: Antioxidants (Basel) Date: 2021-12-15