Elena Rodríguez-Rodríguez1, Aránzazu Aparicio2, Pedro Andrés1, Rosa M Ortega2. 1. Analytical Chemistry Departmental Section, Faculty of Pharmacy, Complutense University from Madrid, Madrid, Spain, and UCM Research Group, Valornut (920030), Complutense University from Madrid, Madrid, Spain. 2. Nutrition Department, Faculty of Pharmacy, Complutense University from Madrid, Madrid, Spain, and UCM Research Group, Valornut (920030), Complutense University from Madrid, Madrid, Spain.
Abstract
OBJECTIVE: Obesity has been associated with vitamin D deficiency and increased oxidative stress, which can lead to the dysregulation of adipokines and inflammation. The aim of the present work was to examine the association of vitamin D status [25(OH)D] on inflammatory related markers in overweight/obese children. SUBJECTS/ METHODS: A total of 137 Spanish schoolchildren between 9 and 12 years of age (31.4% with overweight/obesity) were studied. Being overweight was defined as BMI≥85th percentile and obesity as BMI≥97th percentile using the reference tables of Hernández. Serum 25(OH)D concentrations were measured by chemiluminescent assay. Plasma tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) were measured by immunoenzyme assay. Serum adiponectin was determined using an ELISA kit. Serum high-sensitivity C-reactive protein (hs-PCR) was tested by immunonephelometry. RESULTS: IL-6 concentrations were higher in the overweight/obese children with deficient serum 25(OH)D (<20 ng/mL) than in those in this group but whose serum 25(OH)D concentrations were adequate (≥20 ng/mL). Serum 25(OH)D was inversely associated with IL-6 concentrations in the overweight/obese subjects taking into account different covariates; thus, for every 1 ng/mL rise in the former, the latter fell by 0.160 pg/mL (β=-0.160±0.068; R2=0.131; p=0.023). The obese subjects with concentrations of ≥25 ng/mL had lower hs-CRP values compared to those with concentrations of <25 ng/mL (0.053±0.035 vs. 0.356±0.613 mg/dL; p=0.035). CONCLUSION: Low serum 25(OH)D was significantly associated high serum IL-6 in overweight/obese children, and with increased hs-CRP in obese children.
OBJECTIVE:Obesity has been associated with vitamin Ddeficiency and increased oxidative stress, which can lead to the dysregulation of adipokines and inflammation. The aim of the present work was to examine the association of vitamin D status [25(OH)D] on inflammatory related markers in overweight/obesechildren. SUBJECTS/ METHODS: A total of 137 Spanish schoolchildren between 9 and 12 years of age (31.4% with overweight/obesity) were studied. Being overweight was defined as BMI≥85th percentile and obesity as BMI≥97th percentile using the reference tables of Hernández. Serum 25(OH)D concentrations were measured by chemiluminescent assay. Plasma tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) were measured by immunoenzyme assay. Serum adiponectin was determined using an ELISA kit. Serum high-sensitivity C-reactive protein (hs-PCR) was tested by immunonephelometry. RESULTS:IL-6 concentrations were higher in the overweight/obesechildren with deficient serum 25(OH)D (<20 ng/mL) than in those in this group but whose serum 25(OH)D concentrations were adequate (≥20 ng/mL). Serum 25(OH)D was inversely associated with IL-6 concentrations in the overweight/obese subjects taking into account different covariates; thus, for every 1 ng/mL rise in the former, the latter fell by 0.160 pg/mL (β=-0.160±0.068; R2=0.131; p=0.023). The obese subjects with concentrations of ≥25 ng/mL had lower hs-CRP values compared to those with concentrations of <25 ng/mL (0.053±0.035 vs. 0.356±0.613 mg/dL; p=0.035). CONCLUSION: Low serum 25(OH)D was significantly associated high serum IL-6 in overweight/obesechildren, and with increased hs-CRP in obesechildren.
Entities:
Keywords:
child; inflammation; obesity; overweight; vitamin D
Authors: Maria Krajewska; Ewelina Witkowska-Sędek; Małgorzata Rumińska; Anna Stelmaszczyk-Emmel; Maria Sobol; Anna Majcher; Beata Pyrżak Journal: Front Endocrinol (Lausanne) Date: 2022-06-13 Impact factor: 6.055