Elysia M S Tan1, Daria A Chudakova1, Paul F Davis1, Helen D Brasch1, Tinte Itinteang1, Swee T Tan2. 1. Gillies McIndoe Research Institute, Wellington, New Zealand. 2. Gillies McIndoe Research Institute, Wellington, New Zealand Centre for the Study & Treatment of Vascular Birthmarks, Wellington Regional Plastic, Maxillofacial & Burns Unit, Hutt Hospital, Wellington, New Zealand.
Abstract
AIMS: Cells expressing markers of mast cells, macrophages and dendritic cells have previously been demonstrated within the interstitium of infantile haemangioma (IH). This study characterised these myeloid cellular subpopulations within IH. METHODS: Immunohistochemical staining was performed on proliferating and involuted IHs for the expression of Nanog, tryptase, CD163, DC-SIGN and CD45. The presence of mRNA corresponding to Nanog, tryptase α/β-1, tryptase β-2, CD163 and DC-SIGN was confirmed by NanoString and RT-PCR in snap-frozen IH tissues. RESULTS: Immunohistochemical staining showed expression of Nanog by interstitial phenotypical mast cells within proliferating IH, which were separate from the interstitial M2-polarised macrophages that also expressed DC-SIGN, a dendritic cell marker. These two myeloid cellular subpopulations in IH did not express the pan-haematopoietic marker, CD45. CONCLUSIONS: There are two interstitial subpopulations of myeloid cells within IH: phenotypical mast cells which also express Nanog, indicating a primitive phenotype; and M2-polarised macrophages which also express DC-SIGN. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.
AIMS: Cells expressing markers of mast cells, macrophages and dendritic cells have previously been demonstrated within the interstitium of infantile haemangioma (IH). This study characterised these myeloid cellular subpopulations within IH. METHODS: Immunohistochemical staining was performed on proliferating and involuted IHs for the expression of Nanog, tryptase, CD163, DC-SIGN and CD45. The presence of mRNA corresponding to Nanog, tryptase α/β-1, tryptase β-2, CD163 and DC-SIGN was confirmed by NanoString and RT-PCR in snap-frozen IH tissues. RESULTS: Immunohistochemical staining showed expression of Nanog by interstitial phenotypical mast cells within proliferating IH, which were separate from the interstitial M2-polarised macrophages that also expressed DC-SIGN, a dendritic cell marker. These two myeloid cellular subpopulations in IH did not express the pan-haematopoietic marker, CD45. CONCLUSIONS: There are two interstitial subpopulations of myeloid cells within IH: phenotypical mast cells which also express Nanog, indicating a primitive phenotype; and M2-polarised macrophages which also express DC-SIGN. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.
Authors: Helen H Yu; Therese Featherston; Swee T Tan; Alice M Chibnall; Helen D Brasch; Paul F Davis; Tinte Itinteang Journal: Front Surg Date: 2016-08-02
Authors: Therese Featherston; Helen H Yu; Jonathan C Dunne; Alice M Chibnall; Helen D Brasch; Paul F Davis; Swee T Tan; Tinte Itinteang Journal: Front Surg Date: 2016-09-27
Authors: Tinte Itinteang; Alice M Chibnall; Reginald Marsh; Jonathan C Dunne; Sophie de Jong; Paul F Davis; Philip Leadbitter; Swee T Tan Journal: Front Surg Date: 2016-02-09
Authors: Amy Ruth Bradshaw; Agadha Crisantha Wickremesekera; Helen D Brasch; Alice M Chibnall; Paul F Davis; Swee T Tan; Tinte Itinteang Journal: Front Surg Date: 2016-09-27
Authors: Hugo N Humphries; Susrutha K Wickremesekera; Reginald W Marsh; Helen D Brasch; Shreeja Mehrotra; Swee T Tan; Tinte Itinteang Journal: Front Surg Date: 2018-01-22
Authors: Ranui Baillie; Tinte Itinteang; Helen H Yu; Helen D Brasch; Paul F Davis; Swee T Tan Journal: J Clin Pathol Date: 2016-04-19 Impact factor: 3.411
Authors: Amy Bradshaw; Agadha Wickremesekera; Helen D Brasch; Alice M Chibnall; Paul F Davis; Swee T Tan; Tinte Itinteang Journal: Front Surg Date: 2016-08-26