| Literature DB >> 25828693 |
G Venkatesan1, V Bhanuprakash2, V Balamurugan3.
Abstract
A loop-mediated isothermal amplification (LAMP) assay targeting DNA Pol gene was optimized and evaluated for the rapid detection of orf virus in clinical samples. The LAMP assay was found to be specific and sensitive. The detection rate of LAMP (89.3%) was better than PCR (67.9%) and comparable to real-time PCR (91.1%) in clinical samples by gel electrophoresis and visual detection methods. This LAMP assay is simple and does not rely upon any special equipment and could be employed in clinical diagnosis and epidemiological survey of orf infection.Entities:
Keywords: Diagnosis; HNB dye; LAMP assay; Orf virus; SYBR green I
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Year: 2015 PMID： 25828693 DOI： 10.1016/j.mcp.2015.03.006
Source DB: PubMed Journal: Mol Cell Probes ISSN： 0890-8508 Impact factor: 2.365