Literature DB >> 25827088

Site-specific recombinational cloning using gateway and in-fusion cloning schemes.

Jaehong Park1, Andrea L Throop2, Joshua LaBaer1,2.   

Abstract

The comprehensive study of protein structure and function, or proteomics, depends on the obtainability of full-length cDNAs in species-specific expression vectors and subsequent functional analysis of the expressed protein. Recombinational cloning is a universal cloning technique based on site-specific recombination that is independent of the insert DNA sequence of interest, which differentiates it from classical restriction enzyme-based cloning methods. Recombinational cloning enables rapid and efficient parallel transfer of DNA inserts into multiple expression systems. This unit summarizes strategies for generating expression-ready clones using the most popular commercial recombinational cloning technologies, Gateway (Life Technologies) and In-Fusion (Clontech).
Copyright © 2015 John Wiley & Sons, Inc.

Entities:  

Keywords:  Cre-Lox; Gateway; In-Fusion; high-throughput; recombinational cloning

Mesh：

Substances：

Year:  2015        PMID： 25827088      PMCID： PMC4492480          DOI： 10.1002/0471142727.mb0320s110

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  27 in total

1.  In-fusion assembly: seamless engineering of multidomain fusion proteins, modular vectors, and mutations.

Authors:  Baogong Zhu; Guifang Cai; Emily O Hall; Gordon J Freeman
Journal:  Biotechniques       Date:  2007-09       Impact factor: 1.993

2.  Enzymatic assembly of DNA molecules up to several hundred kilobases.

Authors:  Daniel G Gibson; Lei Young; Ray-Yuan Chuang; J Craig Venter; Clyde A Hutchison; Hamilton O Smith
Journal:  Nat Methods       Date:  2009-04-12       Impact factor: 28.547

3.  In-fusion® cloning with vaccinia virus DNA polymerase.

Authors:  Chad R Irwin; Andrew Farmer; David O Willer; David H Evans
Journal:  Methods Mol Biol       Date:  2012

4.  Systematic analysis of the IgG antibody immune response against varicella zoster virus (VZV) using a self-assembled protein microarray.

Authors:  Alessandro Ceroni; Sahar Sibani; Armin Baiker; Venkata Raveendra Pothineni; Susanne M Bailer; Joshua LaBaer; Jürgen Haas; Colin J Campbell
Journal:  Mol Biosyst       Date:  2010-06-01

5.  High throughput construction and small scale expression screening of multi-tag vectors in Escherichia coli.

Authors:  Louise E Bird
Journal:  Methods       Date:  2011-08-10       Impact factor: 3.608

Review 6.  High-throughput cloning and expression library creation for functional proteomics.

Authors:  Fernanda Festa; Jason Steel; Xiaofang Bian; Joshua Labaer
Journal:  Proteomics       Date:  2013-04-05       Impact factor: 3.984

7.  Production and sequence validation of a complete full length ORF collection for the pathogenic bacterium Vibrio cholerae.

Authors:  Andreas Rolfs; Wagner R Montor; Sang Sun Yoon; Yanhui Hu; Bhupinder Bhullar; Fontina Kelley; Seamus McCarron; Daniel A Jepson; Binghua Shen; Elena Taycher; Stephanie E Mohr; Dongmei Zuo; Janice Williamson; John Mekalanos; Joshua Labaer
Journal:  Proc Natl Acad Sci U S A       Date:  2008-03-12       Impact factor: 11.205

8.  A full-genomic sequence-verified protein-coding gene collection for Francisella tularensis.

Authors:  Tal Murthy; Andreas Rolfs; Yanhui Hu; Zhenwei Shi; Jacob Raphael; Donna Moreira; Fontina Kelley; Seamus McCarron; Daniel Jepson; Elena Taycher; Dongmei Zuo; Stephanie E Mohr; Mauricio Fernandez; Leonardo Brizuela; Joshua LaBaer
Journal:  PLoS One       Date:  2007-06-27       Impact factor: 3.240

9.  Vibrio cholerae proteome-wide screen for immunostimulatory proteins identifies phosphatidylserine decarboxylase as a novel Toll-like receptor 4 agonist.

Authors:  Ann Thanawastien; Wagner R Montor; Joshua Labaer; John J Mekalanos; Sang Sun Yoon
Journal:  PLoS Pathog       Date:  2009-08-21       Impact factor: 6.823

10.  A biomedically enriched collection of 7000 human ORF clones.

Authors:  Andreas Rolfs; Yanhui Hu; Lars Ebert; Dietmar Hoffmann; Dongmei Zuo; Niro Ramachandran; Jacob Raphael; Fontina Kelley; Seamus McCarron; Daniel A Jepson; Binghua Shen; Munira M A Baqui; Joseph Pearlberg; Elena Taycher; Craig DeLoughery; Andreas Hoerlein; Bernhard Korn; Joshua LaBaer
Journal:  PLoS One       Date:  2008-01-30       Impact factor: 3.240
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  9 in total

1.  Identification of Antibody Targets for Tuberculosis Serology using High-Density Nucleic Acid Programmable Protein Arrays.

Authors:  Lusheng Song; Garrick Wallstrom; Xiaobo Yu; Marika Hopper; Jennifer Van Duine; Jason Steel; Jin Park; Peter Wiktor; Peter Kahn; Al Brunner; Douglas Wilson; Elizabeth R Jenny-Avital; Ji Qiu; Joshua Labaer; D Mitchell Magee; Jacqueline M Achkar
Journal:  Mol Cell Proteomics       Date:  2017-02-21       Impact factor: 5.911

2.  A Recombinant HAV Expressing a Neutralization Epitope of HEV Induces Immune Response against HAV and HEV in Mice.

Authors:  Kui Xiang; Yuri Kusov; Guan Ying; Wang Yan; Yi Shan; Wu Jinyuan; Yin Na; Zhou Yan; Li Hongjun; Sun Maosheng
Journal:  Viruses       Date:  2017-09-15       Impact factor: 5.048

3.  AAV-Mediated Astrocyte-Specific Gene Expression under Human ALDH1L1 Promoter in Mouse Thalamus.

Authors:  Wuhyun Koh; Yongmin Mason Park; Seung Eun Lee; C Justin Lee
Journal:  Exp Neurobiol       Date:  2017-12-15       Impact factor: 3.261

4.  Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system.

Authors:  Roman J Szczesny; Katarzyna Kowalska; Kamila Klosowska-Kosicka; Aleksander Chlebowski; Ewelina P Owczarek; Zbigniew Warkocki; Tomasz M Kulinski; Dorota Adamska; Kamila Affek; Agata Jedroszkowiak; Anna V Kotrys; Rafal Tomecki; Pawel S Krawczyk; Lukasz S Borowski; Andrzej Dziembowski
Journal:  PLoS One       Date:  2018-03-28       Impact factor: 3.240

5.  Expression, purification, and characterisation of human soluble Epoxide Hydrolase (hsEH) and of its functional C-terminal domain.

Authors:  Giancarlo Abis; Rebecca L Charles; Philip Eaton; Maria R Conte
Journal:  Protein Expr Purif       Date:  2018-09-12       Impact factor: 1.650

Review 6.  Advances in the Production and Batch Reformatting of Phage Antibody Libraries.

Authors:  Rose H Reader; Robert G Workman; Ben C Maddison; Kevin C Gough
Journal:  Mol Biotechnol       Date:  2019-11       Impact factor: 2.695

7.  Isolation of Monoclonal Antibodies from Zika Virus-Infected Patient Samples.

Authors:  Qihui Wang; Jinghua Yan
Journal:  Methods Mol Biol       Date:  2020

8.  Unveiling the dimer/monomer propensities of Smad MH1-DNA complexes.

Authors:  Lidia Ruiz; Zuzanna Kaczmarska; Tiago Gomes; Eric Aragon; Carles Torner; Regina Freier; Blazej Baginski; Pau Martin-Malpartida; Natàlia de Martin Garrido; José A Marquez; Tiago N Cordeiro; Radoslaw Pluta; Maria J Macias
Journal:  Comput Struct Biotechnol J       Date:  2021-01-06       Impact factor: 7.271

9.  Tomato ATP-Binding Cassette Transporter SlABCB4 Is Involved in Auxin Transport in the Developing Fruit.

Authors:  Peter Amoako Ofori; Markus Geisler; Martin di Donato; Hao Pengchao; Shungo Otagaki; Shogo Matsumoto; Katsuhiro Shiratake
Journal:  Plants (Basel)       Date:  2018-08-13
  9 in total

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