| Literature DB >> 25825286 |
Liang Yan1, Xiao Wang2, Hui Liu2, Yang Tian3, Jinmin Lian4, Ruijuan Yang5, Shumei Hao6, Xuanjun Wang7, Shengchao Yang7, Qiye Li4, Shuai Qi8, Ling Kui2, Moses Okpekum2, Xiao Ma7, Jiajin Zhang9, Zhaoli Ding2, Guojie Zhang4, Wen Wang2, Yang Dong10, Jun Sheng11.
Abstract
Dendrobium officinale Kimura et Migo is a traditional Chinese orchid herb that has both ornamental value and a broad range of therapeutic effects. Here, we report the first de novo assembled 1.35 Gb genome sequences for D. officinale by combining the second-generation Illumina Hiseq 2000 and third-generation PacBio sequencing technologies. We found that orchids have a complete inflorescence gene set and have some specific inflorescence genes. We observed gene expansion in gene families related to fungus symbiosis and drought resistance. We analyzed biosynthesis pathways of medicinal components of D. officinale and found extensive duplication of SPS and SuSy genes, which are related to polysaccharide generation, and that the pathway of D. officinale alkaloid synthesis could be extended to generate 16-epivellosimine. The D. officinale genome assembly demonstrates a new approach to deciphering large complex genomes and, as an important orchid species and a traditional Chinese medicine, the D. officinale genome will facilitate future research on the evolution of orchid plants, as well as the study of medicinal components and potential genetic breeding of the dendrobe.Entities:
Keywords: Dendrobium officinale; Illunima; PacBio; genome; orchid plant
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Year: 2014 PMID： 25825286 DOI： 10.1016/j.molp.2014.12.011
Source DB: PubMed Journal: Mol Plant ISSN： 1674-2052 Impact factor: 13.164