Bacillus wuyishanensis sp. nov., isolated from rhizosphere soil of a medical plant, Prunella vulgaris.

A Gram-staining-positive, rod-shaped, endospore-forming, aerobic bacterium (FJAT-17212(T)) was isolated from the rhizosphere soil of a medical plant, Prunella vulgaris (common selfheal), on the Wuyishan mountain of China. Isolate FJAT-17212(T) grew at 10-50 °C (optimum 30 °C), pH 5-11 (optimum pH 7) and with 0-6% (w/v) NaCl (optimum 2%). Phylogenetic analyses based on 16S rRNA gene sequences showed that isolate FJAT-17212(T) was a member of the genus Bacillus and was most closely related to Bacillus galactosidilyticus DSM 15595(T) (97.3%). DNA-DNA relatedness between isolate FJAT-17212(T) and B. galactosidilyticus DSM 15595(T) was low (35.2% ± 2.3). The diagnostic diamino acid of the peptidoglycan of isolate FJAT-17212(T) was meso-diaminopimelic acid and the predominant isoprenoid quinone was MK-7 (80.8%). The major cellular fatty acids were iso-C15 : 0 (35.7%), anteiso-C15 : 0 (29.8%), iso-C14 : 0 (9.9%) and iso-C16 : 0 (9.9%) and the DNA G+C content was 39.8 mol%. Phenotypic, chemotaxonomic and genotypic properties clearly indicated that isolate FJAT-17212(T) represents a novel species within the genus Bacillus, for which the name Bacillus wuyishanensis sp. nov. is proposed. The type strain is FJAT-17212(T) ( = DSM 27848(T) = CGMCC 1.12709(T)).