Literature DB >> 25825118

A new heterologous fibrin sealant as scaffold to recombinant human bone morphogenetic protein-2 (rhBMP-2) and natural latex proteins for the repair of tibial bone defects.

Eduardo Gomes Machado1, João Paulo Mardegan Issa2, Fellipe Augusto Tocchini de Figueiredo2, Geovane Ribeiro Dos Santos1, Ewerton Alexandre Galdeano1, Mariana Carla Alves1, Erivelto Luis Chacon1, Rui Seabra Ferreira Junior3, Benedito Barraviera4, Marcelo Rodrigues da Cunha5.   

Abstract

Tissue engineering has special interest in bone tissue aiming at future medical applications Studies have focused on recombinant human bone morphogenetic protein-2 (rhBMP-2) and natural latex proteins due to the osteogenic properties of rhBMP-2 and the angiogenic characteristic of fraction 1 protein (P-1) extracted from the rubber tree Hevea brasiliensis. Furthermore, heterologous fibrin sealant (FS) has been shown as a promising alternative in regenerative therapies. The aim of this study was to evaluate these substances for the repair of bone defects in rats. A bone defect measuring 3mm in diameter was created in the proximal metaphysis of the left tibia of 60 rats and was implanted with rhBMP-2 or P-1 in combination with a new heterologous FS derived from snake venom. The animals were divided into six groups: control (unfilled bone defect), rhBMP-2 (defect filled with 5μg rhBMP-2), P-1 (defect filled with 5μg P-1), FS (defect filled with 8μg FS), FS/rhBMP-2 (defect filled with 8μg FS and 5μg rhBMP-2), FS/P-1 (defect filled with 8μg FS and 5μg P-1). The animals were sacrificed 2 and 6 weeks after surgery. The newly formed bone projected from the margins of the original bone and exhibited trabecular morphology and a disorganized arrangement of osteocyte lacunae. Immunohistochemical analysis showed intense expression of osteocalcin in all groups. Histometric analysis revealed a significant difference in all groups after 2 weeks (p<0.05), except for the rhBMP-2 and FS/rhBMP-2 groups (p>0.05). A statistically significant difference (p<0.05) was observed in all groups after 6 weeks in relation to the volume of newly formed bone in the surgical area. In conclusion, the new heterologous fibrin sealant was found to be biocompatible and the combination with rhBMP-2 showed the highest osteogenic and osteoconductive capacity for bone healing. These findings suggest a promising application of this combination in the regeneration surgery.
Copyright © 2015 Elsevier GmbH. All rights reserved.

Entities:  

Keywords:  BMP-2; Fibrin; Natural latex; Protein P-1; Scaffold; Sealant

Mesh：

Substances：

Year:  2015        PMID： 25825118     DOI： 10.1016/j.acthis.2015.03.006

Source DB:  PubMed          Journal:  Acta Histochem        ISSN： 0065-1281            Impact factor:   2.479


  13 in total

1.  The new heterologous fibrin sealant in combination with low-level laser therapy (LLLT) in the repair of the buccal branch of the facial nerve.

Authors:  Daniela Vieira Buchaim; Antonio de Castro Rodrigues; Rogerio Leone Buchaim; Benedito Barraviera; Rui Seabra Ferreira Junior; Geraldo Marco Rosa Junior; Cleuber Rodrigo de Souza Bueno; Domingos Donizeti Roque; Daniel Ventura Dias; Leticia Rossi Dare; Jesus Carlos Andreo
Journal:  Lasers Med Sci       Date:  2016-04-25       Impact factor: 3.161

2.  Suitability of the use of an elastin matrix combined with bone morphogenetic protein for the repair of cranial defects.

Authors:  Renato de Moraes; Ana Maria de Guzzi Plepis; Virginia da Conceição Amaro Martins; Marco Antonio Hungaro Duarte; Murilo Priori Alcalde; Rogerio Leone Buchaim; Karina Torres Pomini; Eduardo Gomes Machado; Marcelo de Azevedo E Sousa Munhoz; Fernando Bento Cunha; Amanda Regina Alves Calegari; Amilton Iatecola; Samantha Ketelyn Silva; Marcelo Rodrigues da Cunha
Journal:  Am J Transl Res       Date:  2019-08-15       Impact factor: 4.060

Review 3.  Heterologous fibrin sealant derived from snake venom: from bench to bedside - an overview.

Authors:  Rui Seabra Ferreira; Luciana Curtolo de Barros; Luciana Patrícia Fernandes Abbade; Silvia Regina Catharino Sartori Barraviera; Maria Regina Cavariani Silvares; Leticia Gomes de Pontes; Lucilene Delazari Dos Santos; Benedito Barraviera
Journal:  J Venom Anim Toxins Incl Trop Dis       Date:  2017-04-04

4.  Nanotechnology for Stimulating Osteoprogenitor Differentiation.

Authors:  A Ibrahim; N W Bulstrode; I S Whitaker; D M Eastwood; D Dunaway; P Ferretti
Journal:  Open Orthop J       Date:  2016-12-30

5.  Fibrin biopolymer as scaffold candidate to treat bone defects in rats.

Authors:  Claudia Vilalva Cassaro; Luis Antonio Justulin; Patrícia Rodrigues de Lima; Marjorie de Assis Golim; Natália Perussi Biscola; Mateus Vidigal de Castro; Alexandre Leite Rodrigues de Oliveira; Danuta Pulz Doiche; Elenize Jamas Pereira; Rui Seabra Ferreira; Benedito Barraviera
Journal:  J Venom Anim Toxins Incl Trop Dis       Date:  2019-11-04

6.  Elastin-derived scaffolding associated or not with bone morphogenetic protein (BMP) or hydroxyapatite (HA) in the repair process of metaphyseal bone defects.

Authors:  Marcelo de Azevedo E Sousa Munhoz; Karina Torres Pomini; Ana Maria de Guzzi Plepis; Virginia da Conceição Amaro Martins; Eduardo Gomes Machado; Renato de Moraes; Fernando Bento Cunha; Arnaldo Rodrigues Santos Junior; Guinea Brasil Camargo Cardoso; Marco Antonio Hungaro Duarte; Murilo Priori Alcalde; Daniela Vieira Buchaim; Rogerio Leone Buchaim; Marcelo Rodrigues da Cunha
Journal:  PLoS One       Date:  2020-04-20       Impact factor: 3.240

7.  Photobiomodulation Therapy Associated with Heterologous Fibrin Biopolymer and Bovine Bone Matrix Helps to Reconstruct Long Bones.

Authors:  Marcelie Priscila de Oliveira Rosso; Aline Tiemi Oyadomari; Karina Torres Pomini; Bruna Botteon Della Coletta; João Vitor Tadashi Cosin Shindo; Rui Seabra Ferreira Júnior; Benedito Barraviera; Claudia Vilalva Cassaro; Daniela Vieira Buchaim; Daniel de Bortoli Teixeira; Sandra Maria Barbalho; Murilo Priori Alcalde; Marco Antonio Hungaro Duarte; Jesus Carlos Andreo; Rogério Leone Buchaim
Journal:  Biomolecules       Date:  2020-03-02

8.  Three-dimensional environment and vascularization induce osteogenic maturation of human adipose-derived stem cells comparable to that of bone-derived progenitors.

Authors:  Amel Ibrahim; Naiara Rodriguez-Florez; Oliver F W Gardner; Eleonora Zucchelli; Sophie E P New; Alessandro Borghi; David Dunaway; Neil W Bulstrode; Patrizia Ferretti
Journal:  Stem Cells Transl Med       Date:  2020-07-08       Impact factor: 6.940

9.  A unique heterologous fibrin sealant (HFS) as a candidate biological scaffold for mesenchymal stem cells in osteoporotic rats.

Authors:  Patrícia Rodrigues Orsi; Fernanda Cruz Landim-Alvarenga; Luis Antônio Justulin; Ramon Kaneno; Marjorie de Assis Golim; Daniela Carvalho Dos Santos; Camila Fernanda Zorzella Creste; Eunice Oba; Leandro Maia; Benedito Barraviera; Rui Seabra Ferreira
Journal:  Stem Cell Res Ther       Date:  2017-09-29       Impact factor: 6.832

10.  Use of an anionic collagen matrix made from bovine intestinal serosa for in vivo repair of cranial defects.

Authors:  Mariane Silva Pettian; Ana Maria de Guzzi Plepis; Virginia da Conceição Amaro Martins; Geovane Ribeiro Dos Santos; Clovis Antônio Lopes Pinto; Ewerton Alexandre Galdeano; Amanda Regina Alves Calegari; Carlos Alberto de Moraes; Marcelo Rodrigues da Cunha
Journal:  PLoS One       Date:  2018-07-12       Impact factor: 3.240
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