Literature DB >> 2582350

DNaseI sensitivity of the rat albumin and alpha-fetoprotein genes.

L Kunnath, J Locker.   

Abstract

We have analyzed the DNaseI sensitivity of chromatin from the rat albumin and alpha-fetoprotein genes in the fetal liver (which synthesizes albumin and alpha-fetoprotein), adult liver (which synthesizes albumin), fetal yolk sac (which synthesizes alpha-fetoprotein), and adult kidney (which synthesizes neither). Active genes were much more sensitive than their kidney counterparts, and the adult liver alpha-fetoprotein and fetal yolk sac albumin genes showed intermediate levels of sensitivity. Sensitivity was analyzed as a function of the extent of DNaseI digestion. Rate constants were calculated for the degradation of individual DNA hybridization bands and normalized to the intrinsic rate constants of the same bands degraded in purified DNA. This enabled us to eliminate the inconsistencies that otherwise result from comparing chromatin sensitivity of different DNA sequences, or chromatin sensitivity in different nuclear environments.

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Year:  1985        PMID： 2582350      PMCID： PMC340978          DOI： 10.1093/nar/13.1.115

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  32 in total

1.  The rat serum albumin gene: analysis of cloned sequences.

Authors:  T D Sargent; J R Wu; J M Sala-Trepat; R B Wallace; A A Reyes; J Bonner
Journal:  Proc Natl Acad Sci U S A       Date:  1979-07       Impact factor: 11.205

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.

Authors:  J C Alwine; D J Kemp; B A Parker; J Reiser; J Renart; G R Stark; G M Wahl
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

4.  The chromatin structure of specific genes: II. Disruption of chromatin structure during gene activity.

Authors:  C Wu; Y C Wong; S C Elgin
Journal:  Cell       Date:  1979-04       Impact factor: 41.582

5.  The chromatin structure of specific genes: I. Evidence for higher order domains of defined DNA sequence.

Authors:  C Wu; P M Bingham; K J Livak; R Holmgren; S C Elgin
Journal:  Cell       Date:  1979-04       Impact factor: 41.582

Review 6.  DNAase I-hypersensitive sites of chromatin.

Authors:  S C Elgin
Journal:  Cell       Date:  1981-12       Impact factor: 41.582

7.  Isolation of a subclass of nuclear proteins responsible for conferring a DNase I-sensitive structure on globin chromatin.

Authors:  S Weisbrod; H Weintraub
Journal:  Proc Natl Acad Sci U S A       Date:  1979-02       Impact factor: 11.205

Review 8.  DNA methylation and gene function.

Authors:  A Razin; A D Riggs
Journal:  Science       Date:  1980-11-07       Impact factor: 47.728

9.  Developmental regulation of albumin and alpha-fetoprotein gene expression in the rat.

Authors:  L Muglia; J Locker
Journal:  Nucleic Acids Res       Date:  1984-09-11       Impact factor: 16.971

10.  Correlated morphometric and biochemical studies on the liver cell. I. Morphometric model, stereologic methods, and normal morphometric data for rat liver.

Authors:  E R Weibel; W Stäubli; H R Gnägi; F A Hess
Journal:  J Cell Biol       Date:  1969-07       Impact factor: 10.539
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  6 in total

1.  Genes expressed in cortical neurons--chromatin conformation and DNase I hypersensitive sites.

Authors:  T R Ivanov; I R Brown
Journal:  Neurochem Res       Date:  1989-02       Impact factor: 3.996

2.  DNase I-hypersensitive sites in the 5'-flanking region of the rat serum albumin gene: correlation between chromatin structure and transcriptional activity.

Authors:  L E Babiss; A Bennett; J M Friedman; J E Darnell
Journal:  Proc Natl Acad Sci U S A       Date:  1986-09       Impact factor: 11.205

3.  Sequence conservation and structural organization of the glycerol-3-phosphate dehydrogenase promoter in mice and humans.

Authors:  B Gwynn; K A Lyford; E H Birkenmeier
Journal:  Mol Cell Biol       Date:  1990-10       Impact factor: 4.272

Review 4.  A histochemical approach to the knowledge about the neuron nucleus: the "pre-alarm chromatin".

Authors:  M G Manfredi-Romanini
Journal:  Neurochem Res       Date:  1994-06       Impact factor: 3.996

5.  Specific sets of DNase I-hypersensitive sites are associated with the potential and overt expression of the rat albumin and alpha-fetoprotein genes.

Authors:  J L Nahon; A Venetianer; J M Sala-Trepat
Journal:  Proc Natl Acad Sci U S A       Date:  1987-04       Impact factor: 11.205

6.  DNase I hypersensitivity and methylation of the 5'-flanking region of the alpha 1-fetoprotein gene during developmental and glucocorticoid-induced repression of its activity in rat liver.

Authors:  B Turcotte; M Guertin; M Chevrette; H LaRue; L Bélanger
Journal:  Nucleic Acids Res       Date:  1986-12-22       Impact factor: 16.971
  6 in total

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