| Literature DB >> 25822732 |
Nathalie Matusiak1, Aren van Waarde2, Dennie Rozeveld3, Antoon J M van Oosterhout3, Irene H Heijink3, Riccardo Castelli4, Herman S Overkleeft4, Rainer Bischoff5, Rudi A J O Dierckx2, Philip H Elsinga2.
Abstract
Matrix metalloproteinases (MMPs) are the main proteolytic enzymes involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). A radiolabeled MMP inhibitor, [(18)F]FB-ML5, was prepared, and its in vivo kinetics were tested in a mouse model of pulmonary inflammation. BALB/c mice were exposed for 4 days to cigarette smoke (CS) or air. On the fifth day, a dynamic microPET scan was made with [(18)F]FB-ML5. Standardized uptake values (PET-SUVmean) were 0.19 ± 0.06 in the lungs of CS-exposed mice (n = 6) compared to 0.11 ± 0.03 (n = 5) in air-exposed controls (p < 0.05), 90 min post-injection MMP-9 levels in bronchoalveolar lavage fluid (BALF) were increased from undetectable level to 4615 ± 1963 pg/ml by CS exposure. Increased MMP expression in a COPD mouse model was shown to lead to increased retention of [(18)F]FB-ML5.Entities:
Keywords: BALF; COPD; Lung imaging; MMP/ADAM inhibitor; MicroPET
Mesh：
Substances：
Year: 2015 PMID： 25822732 DOI： 10.1007/s11307-015-0847-3
Source DB: PubMed Journal: Mol Imaging Biol ISSN： 1536-1632 Impact factor: 3.488