| Literature DB >> 25821910 |
Shohei Furutachi1, Hiroaki Miya1, Tomoyuki Watanabe1, Hiroki Kawai1, Norihiko Yamasaki1, Yujin Harada1, Itaru Imayoshi2, Mark Nelson3, Keiichi I Nakayama4, Yusuke Hirabayashi1, Yukiko Gotoh1.
Abstract
The mechanism by which adult neural stem cells (NSCs) are established during development is unclear. In this study, analysis of cell cycle progression by examining retention of a histone 2B (H2B)-GFP fusion protein revealed that, in a subset of mouse embryonic neural progenitor cells (NPCs), the cell cycle slows between embryonic day (E) 13.5 and E15.5 while other embryonic NPCs continue to divide rapidly. By allowing H2B-GFP expressed at E9.5 to become diluted in dividing cells until the young adult stage, we determined that a majority of NSCs in the young adult subependymal zone (SEZ) originated from these slowly dividing embryonic NPCs. The cyclin-dependent kinase inhibitor p57 is highly expressed in this embryonic subpopulation, and the deletion of p57 impairs the emergence of adult NSCs. Our results suggest that a substantial fraction of adult SEZ NSCs is derived from a slowly dividing subpopulation of embryonic NPCs and identify p57 as a key factor in generating this embryonic origin of adult SEZ NSCs.Entities:
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Year: 2015 PMID： 25821910 DOI： 10.1038/nn.3989
Source DB: PubMed Journal: Nat Neurosci ISSN： 1097-6256 Impact factor: 24.884