T A L Wagemaker1,2, P Rijo2, L M Rodrigues2,3, P M B G Maia Campos1, A S Fernandes2, C Rosado2. 1. Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil. 2. CBIOS, Universidade Lusófona Research Center in Biosciences & Health Technologies, Lisboa, Portugal. 3. Pharmacological Sciences Department, School of Pharmacy, Universidade de Lisboa, Lisboa, Portugal.
Abstract
OBJECTIVE: Green coffee oil (GCO) has been used in cosmetic formulations due to its emollient and anti-ageing properties. However, there are insufficient studies about its safety when applied in cosmetic formulations. METHODS: Cytotoxicity of GCO and of formulations containing 2.5-15% of GCO was evaluated by the MTT reduction assay, in human keratinocytes. Formulations containing 15% of GCO and the vehicle were applied under in use conditions in the volar forearm of human volunteers during 3 days. Transepidermal water loss, stratum corneum water content and erythema index were evaluated each 24 h using biophysical techniques. The same formulations were probed for skin tolerance through a patch test. RESULTS: Neither pure GCO nor its formulations showed cytotoxic effects in concentrations up to 100 μg mL(-1) . Transepidermal water loss values showed a slight reduction when the formulation containing GCO was applied. Stratum corneum water content and erythema index did not show significant differences, as the results observed in the first day of the study were maintained throughout 3 days. None of the volunteers display any reaction after using an occlusive patch. CONCLUSION: The results obtained in the study indicate that GCO seems to be safe for topical applications and showed good skin compatibility under the experimental conditions of the study.
OBJECTIVE:Green coffee oil (GCO) has been used in cosmetic formulations due to its emollient and anti-ageing properties. However, there are insufficient studies about its safety when applied in cosmetic formulations. METHODS:Cytotoxicity of GCO and of formulations containing 2.5-15% of GCO was evaluated by the MTT reduction assay, in human keratinocytes. Formulations containing 15% of GCO and the vehicle were applied under in use conditions in the volar forearm of human volunteers during 3 days. Transepidermal water loss, stratum corneum water content and erythema index were evaluated each 24 h using biophysical techniques. The same formulations were probed for skin tolerance through a patch test. RESULTS: Neither pure GCO nor its formulations showed cytotoxic effects in concentrations up to 100 μg mL(-1) . Transepidermal water loss values showed a slight reduction when the formulation containing GCO was applied. Stratum corneum water content and erythema index did not show significant differences, as the results observed in the first day of the study were maintained throughout 3 days. None of the volunteers display any reaction after using an occlusive patch. CONCLUSION: The results obtained in the study indicate that GCO seems to be safe for topical applications and showed good skin compatibility under the experimental conditions of the study.
Authors: Bruno Grosselli Lania; Joseane Morari; Aglécio Luis de Souza; Marilene Neves da Silva; Amanda Roberta de Almeida; Gislaine Veira-Damiani; Sarah Monte Alegre; Carlos Lenz César; Lício Augusto Velloso; Maria Letícia Cintra; Nilson Borlina Maia; Paulo Eduardo Neves Ferreira Velho Journal: PLoS One Date: 2017-12-13 Impact factor: 3.240
Authors: Ana Júlio; Rita Caparica; Sofia A Costa Lima; Ana Sofia Fernandes; Catarina Rosado; Duarte M F Prazeres; Salette Reis; Tânia Santos de Almeida; Pedro Fonte Journal: Nanomaterials (Basel) Date: 2019-08-10 Impact factor: 5.076
Authors: Marisa Nicolai; Joana Mota; Ana S Fernandes; Filipe Pereira; Paula Pereira; Catarina P Reis; Maria Valéria Robles Velasco; André Rolim Baby; Catarina Rosado; Patrícia Rijo Journal: Pharmaceuticals (Basel) Date: 2020-06-10