| Literature DB >> 25815779 |
Mariana Amaro1, Jan Brezovský2,3, Silvia Kováčová4,3, Jan Sýkora1, David Bednář2,3, Václav Němec4,3, Veronika Lišková2, Nagendra Prasad Kurumbang2, Koen Beerens2, Radka Chaloupková2, Kamil Paruch4,3, Martin Hof1, Jiří Damborský2,3.
Abstract
Hydration of proteins profoundly affects their functions. We describe a simple and general method for site-specific analysis of protein hydration based on the in vivo incorporation of fluorescent unnatural amino acids and their analysis by steady-state fluorescence spectroscopy. Using this method, we investigate the hydration of functionally important regions of dehalogenases. The experimental results are compared to findings from molecular dynamics simulations.Mesh：
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Year: 2015 PMID： 25815779 DOI： 10.1021/jacs.5b01681
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419