AIM: These studies were to validate the analytical performance of a gene expression signature that differentiates melanoma and nevi, using RNA expression from 14 signature genes and nine normalization genes that generates a melanoma diagnostic score (MDS). MATERIALS & METHODS: Formalin-fixed paraffin-embedded melanocytic lesions were evaluated in these studies. RESULTS: The overall SD of the assay was determined to be 0.69 MDS units. Individual amplicons within the signature had an average amplification efficiency of 92% and a SD less than 0.5 CT. The MDS was reproducible across a 2000-fold dilution range of input RNA. Melanin, an inhibitor of PCR, does not interfere with the signature. CONCLUSION: These studies indicate this signature is robust and reproducible and is analytically validated on formalin-fixed paraffin-embedded melanocytic lesions.
AIM: These studies were to validate the analytical performance of a gene expression signature that differentiates melanoma and nevi, using RNA expression from 14 signature genes and nine normalization genes that generates a melanoma diagnostic score (MDS). MATERIALS & METHODS:Formalin-fixed paraffin-embedded melanocytic lesions were evaluated in these studies. RESULTS: The overall SD of the assay was determined to be 0.69 MDS units. Individual amplicons within the signature had an average amplification efficiency of 92% and a SD less than 0.5 CT. The MDS was reproducible across a 2000-fold dilution range of input RNA. Melanin, an inhibitor of PCR, does not interfere with the signature. CONCLUSION: These studies indicate this signature is robust and reproducible and is analytically validated on formalin-fixed paraffin-embedded melanocytic lesions.
Authors: Clay Cockerell; Jaime Tschen; Steven D Billings; Brent Evans; Krystal Brown; Colleen Rock; Loren E Clarke Journal: Per Med Date: 2017-01-23 Impact factor: 2.512
Authors: Loren E Clarke; Darl D Flake; Klaus Busam; Clay Cockerell; Klaus Helm; Jennifer McNiff; Jon Reed; Jaime Tschen; Jinah Kim; Raymond Barnhill; Rosalie Elenitsas; Victor G Prieto; Jonathan Nelson; Hillary Kimbrell; Kathryn A Kolquist; Krystal L Brown; M Bryan Warf; Benjamin B Roa; Richard J Wenstrup Journal: Cancer Date: 2016-10-21 Impact factor: 6.860