AIM: To evaluate the effectiveness of the photodynamic therapy using hypericin (HYP) against both planktonic and biofilm-forming Staphylococcus aureus. MATERIALS & METHODS: HYP photoactivity was evaluated against methicillin-susceptible and resistant S. aureus. Bacterial suspension or biofilm were preincubated with HYP and subjected to LED illumination. Viable bacteria were determined by colony counting. RESULTS: Preincubation with HYP (5 min) plus light exposure (10 min) showed bactericidal effect against planktonic methicillin-susceptible S. aureus and methicillin-resistant S. aureus. Longer preincubation times (24 h) and time light exposure (30 min) were required to reach HYP-photoactivity against S. aureus biofilms. HYP-photoactivity was correlated to the biofilm production. CONCLUSION: HYP could be a potential photosensitizer for the inactivation of staphylococcal biofilms forming on the surfaces accessible to visible light.
AIM: To evaluate the effectiveness of the photodynamic therapy using hypericin (HYP) against both planktonic and biofilm-forming Staphylococcus aureus. MATERIALS & METHODS: HYP photoactivity was evaluated against methicillin-susceptible and resistant S. aureus. Bacterial suspension or biofilm were preincubated with HYP and subjected to LED illumination. Viable bacteria were determined by colony counting. RESULTS: Preincubation with HYP (5 min) plus light exposure (10 min) showed bactericidal effect against planktonic methicillin-susceptible S. aureus and methicillin-resistant S. aureus. Longer preincubation times (24 h) and time light exposure (30 min) were required to reach HYP-photoactivity against S. aureus biofilms. HYP-photoactivity was correlated to the biofilm production. CONCLUSION: HYP could be a potential photosensitizer for the inactivation of staphylococcal biofilms forming on the surfaces accessible to visible light.
Authors: Alice Abu Dayyih; Bernd Gutberlet; Eduard Preis; Konrad H Engelhardt; Muhammad Umair Amin; Ahmed M Abdelsalam; Martina Bonsu; Udo Bakowsky Journal: ACS Appl Mater Interfaces Date: 2022-07-05 Impact factor: 10.383
Authors: Aguinaldo S Garcez; Mohammed Kaplan; Grant J Jensen; Fábio R Scheidt; Eduardo M Oliveira; Selly S Suzuki Journal: Photodiagnosis Photodyn Ther Date: 2020-09-24 Impact factor: 3.631