F J Sutili1, L de Lima Silva2, L T Gressler3, L T Gressler3, E K Battisti4, B M Heinzmann5, A C de Vargas3, B Baldisserotto1. 1. Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, Brazil. 2. Curso de Farmácia, Universidade Regional Integrada do Alto Uruguai e Missões, Santiago, Brazil. 3. Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, Brazil. 4. Departamento de Zootecnia e Ciências Biológicas/CESNORS, Universidade Federal de Santa Maria, Palmeira das Missões, Brazil. 5. Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, Brazil.
Abstract
AIMS: The aims of this study were to investigate the in vitro antibacterial activity of the essential oils (EOs) of Hesperozygis ringens (HREO), popularly known as 'espanta-pulga' and two different species of basil, Ocimum gratissimum (OGEO) and Ocimum americanum (OAEO), as well as, the potential of these products to be used in silver catfish (Rhamdia quelen) infected with Aeromonas hydrophila. METHODS AND RESULTS: OGEO and HREO showed better antibacterial activity in vitro. Subinhibitory concentrations of all EOs inhibited haemolysis caused by Aer. hydrophila in fish erythrocytes (100% reduction for OAEO at 100 μg ml(-1) and more than 90% for HREO and OGEO at 150 μg ml(-1) ). However, OAEO and HREO showed the best survival results (75 and 70% respectively) after their use as treatment (therapeutic baths-1 h daily/5 days) in silver catfish experimentally infected with Aer. hydrophila. A second in vivo assay using healthy fish was conducted to verify the potential of the EOs (preventive baths-1 h daily/5 days) to promote fish survival. Fish exposed to HREO and OAEO and their diluent (ethanol) showed significant lower haematocrit values and higher complement system activity compared to control. Plasma cortisol level was significantly higher in the groups exposed to both EOs. There was no significant difference in survival of silver catfish challenged with Aer. hydrophila after preventive baths with HREO, OAEO and control group. CONCLUSIONS: All tested EOs showed in vitro antibacterial properties against Aer. hydrophila and HREO and OAEO showed potential to be used in the treatment of infected fish. SIGNIFICANCE AND IMPACT OF THE STUDY: These products can be used in aquaculture as therapeutic and prophylactic agents against fish pathogens, with antimicrobial and/or immunostimulant properties.
AIMS: The aims of this study were to investigate the in vitro antibacterial activity of the essential oils (EOs) of Hesperozygis ringens (HREO), popularly known as 'espanta-pulga' and two different species of basil, Ocimum gratissimum (OGEO) and Ocimum americanum (OAEO), as well as, the potential of these products to be used in silver catfish (Rhamdia quelen) infected with Aeromonas hydrophila. METHODS AND RESULTS:OGEO and HREO showed better antibacterial activity in vitro. Subinhibitory concentrations of all EOs inhibited haemolysis caused by Aer. hydrophila in fish erythrocytes (100% reduction for OAEO at 100 μg ml(-1) and more than 90% for HREO and OGEO at 150 μg ml(-1) ). However, OAEO and HREO showed the best survival results (75 and 70% respectively) after their use as treatment (therapeutic baths-1 h daily/5 days) in silver catfish experimentally infected with Aer. hydrophila. A second in vivo assay using healthy fish was conducted to verify the potential of the EOs (preventive baths-1 h daily/5 days) to promote fish survival. Fish exposed to HREO and OAEO and their diluent (ethanol) showed significant lower haematocrit values and higher complement system activity compared to control. Plasma cortisol level was significantly higher in the groups exposed to both EOs. There was no significant difference in survival of silver catfish challenged with Aer. hydrophila after preventive baths with HREO, OAEO and control group. CONCLUSIONS: All tested EOs showed in vitro antibacterial properties against Aer. hydrophila and HREO and OAEO showed potential to be used in the treatment of infected fish. SIGNIFICANCE AND IMPACT OF THE STUDY: These products can be used in aquaculture as therapeutic and prophylactic agents against fish pathogens, with antimicrobial and/or immunostimulant properties.
Authors: Mahmoud A O Dawood; Mohammed F El Basuini; Amr I Zaineldin; Sevdan Yilmaz; Md Tawheed Hasan; Ehsan Ahmadifar; Amel M El Asely; Hany M R Abdel-Latif; Mahmoud Alagawany; Nermeen M Abu-Elala; Hien Van Doan; Hani Sewilam Journal: Pathogens Date: 2021-02-09
Authors: S A Suphoronski; R T Chideroli; C T Facimoto; R M Mainardi; F P Souza; N M Lopera-Barrero; G F A Jesus; M L Martins; G W Di Santis; A de Oliveira; G S Gonçalves; R Dari; S Frouel; U P Pereira Journal: Sci Rep Date: 2019-04-15 Impact factor: 4.379
Authors: Mahmoud A O Dawood; Mohammed F El Basuini; Sevdan Yilmaz; Hany M R Abdel-Latif; Mahmoud Alagawany; Zulhisyam Abdul Kari; Mohammad Khairul Azhar Abdul Razab; Noor Khalidah Abdul Hamid; Tossapol Moonmanee; Hien Van Doan Journal: Animals (Basel) Date: 2022-03-24 Impact factor: 2.752