Literature DB >> 25809245

Improving survival of disassociated human embryonic stem cells by mechanical stimulation using acoustic tweezing cytometry.

Di Chen1, Yubing Sun2, Cheri X Deng3, Jianping Fu4.   

Abstract

Dissociation-induced apoptosis of human embryonic stem cells (hESCs) hampers their large-scale culture. Herein we leveraged the mechanosensitivity of hESCs and employed, to our knowledge, a novel technique, acoustic tweezing cytometry (ATC), for subcellular mechanical stimulation of disassociated single hESCs to improve their survival. By acoustically actuating integrin-bound microbubbles (MBs) to live cells, ATC increased the survival rate and cloning efficiency of hESCs by threefold. A positive correlation was observed between the increased hESC survival rate and total accumulative displacement of integrin-anchored MBs during ATC stimulation. ATC may serve as a promising biocompatible tool to improve hESC culture.
Copyright © 2015 Biophysical Society. Published by Elsevier Inc. All rights reserved.

Entities:  

Mesh：

Substances：

Year:  2015        PMID： 25809245      PMCID： PMC4375556          DOI： 10.1016/j.bpj.2015.01.033

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  11 in total

1.  Individual cell movement, asymmetric colony expansion, rho-associated kinase, and E-cadherin impact the clonogenicity of human embryonic stem cells.

Authors:  Li Li; Bill H Wang; Shuai Wang; Lilian Moalim-Nour; Kanishka Mohib; David Lohnes; Lisheng Wang
Journal:  Biophys J       Date:  2010-06-02       Impact factor: 4.033

2.  Molecular pathway and cell state responsible for dissociation-induced apoptosis in human pluripotent stem cells.

Authors:  Masatoshi Ohgushi; Michiru Matsumura; Mototsugu Eiraku; Kazuhiro Murakami; Toshihiro Aramaki; Ayaka Nishiyama; Keiko Muguruma; Tokushige Nakano; Hidetaka Suga; Morio Ueno; Toshimasa Ishizaki; Hirofumi Suemori; Shuh Narumiya; Hitoshi Niwa; Yoshiki Sasai
Journal:  Cell Stem Cell       Date:  2010-08-06       Impact factor: 24.633

3.  Actin-myosin contractility is responsible for the reduced viability of dissociated human embryonic stem cells.

Authors:  Guokai Chen; Zhonggang Hou; Daniel R Gulbranson; James A Thomson
Journal:  Cell Stem Cell       Date:  2010-08-06       Impact factor: 24.633

4.  Activation of a bacterial mechanosensitive channel in mammalian cells by cytoskeletal stress.

Authors:  Johanna Heureaux; Di Chen; Victoria L Murray; Cheri X Deng; Allen P Liu
Journal:  Cell Mol Bioeng       Date:  2014-09       Impact factor: 2.321

5.  A ROCK inhibitor permits survival of dissociated human embryonic stem cells.

Authors:  Kiichi Watanabe; Morio Ueno; Daisuke Kamiya; Ayaka Nishiyama; Michiru Matsumura; Takafumi Wataya; Jun B Takahashi; Satomi Nishikawa; Shin-ichi Nishikawa; Keiko Muguruma; Yoshiki Sasai
Journal:  Nat Biotechnol       Date:  2007-05-27       Impact factor: 54.908

6.  Soft microenvironments promote the early neurogenic differentiation but not self-renewal of human pluripotent stem cells.

Authors:  Albert J Keung; Prashanth Asuri; Sanjay Kumar; David V Schaffer
Journal:  Integr Biol (Camb)       Date:  2012-08-02       Impact factor: 2.192

7.  Kinetic analysis of cell spreading. I. Theory and modelling of curves.

Authors:  W G Bardsley; J D Aplin
Journal:  J Cell Sci       Date:  1983-05       Impact factor: 5.285

8.  Mechanics regulates fate decisions of human embryonic stem cells.

Authors:  Yubing Sun; Luis G Villa-Diaz; Raymond H W Lam; Weiqiang Chen; Paul H Krebsbach; Jianping Fu
Journal:  PLoS One       Date:  2012-05-16       Impact factor: 3.240

9.  Time-lapse analysis of human embryonic stem cells reveals multiple bottlenecks restricting colony formation and their relief upon culture adaptation.

Authors:  Ivana Barbaric; Veronica Biga; Paul J Gokhale; Mark Jones; Dylan Stavish; Adam Glen; Daniel Coca; Peter W Andrews
Journal:  Stem Cell Reports       Date:  2014-06-12       Impact factor: 7.765

10.  Hippo/YAP-mediated rigidity-dependent motor neuron differentiation of human pluripotent stem cells.

Authors:  Yubing Sun; Koh Meng Aw Yong; Luis G Villa-Diaz; Xiaoli Zhang; Weiqiang Chen; Renee Philson; Shinuo Weng; Haoxing Xu; Paul H Krebsbach; Jianping Fu
Journal:  Nat Mater       Date:  2014-04-13       Impact factor: 43.841
View more
  6 in total

Review 1.  Biophysical Tools for Cellular and Subcellular Mechanical Actuation of Cell Signaling.

Authors:  Allen P Liu
Journal:  Biophys J       Date:  2016-07-25       Impact factor: 4.033

2.  Acoustic tweezing cytometry enhances osteogenesis of human mesenchymal stem cells through cytoskeletal contractility and YAP activation.

Authors:  Xufeng Xue; Xiaowei Hong; Zida Li; Cheri X Deng; Jianping Fu
Journal:  Biomaterials       Date:  2017-04-22       Impact factor: 12.479

3.  Probing Single-Cell Mechanical Allostasis Using Ultrasound Tweezers.

Authors:  Weiyi Qian; Weiqiang Chen
Journal:  Cell Mol Bioeng       Date:  2019-06-13       Impact factor: 2.321

4.  Acoustic Tweezing Cytometry Induces Rapid Initiation of Human Embryonic Stem Cell Differentiation.

Authors:  Tuğba Topal; Xiaowei Hong; Xufeng Xue; Zhenzhen Fan; Ninad Kanetkar; Joe T Nguyen; Jianping Fu; Cheri X Deng; Paul H Krebsbach
Journal:  Sci Rep       Date:  2018-08-28       Impact factor: 4.379

5.  Acoustic tweezing cytometry for mechanical phenotyping of macrophages and mechanopharmaceutical cytotripsy.

Authors:  Xiaowei Hong; Phillip M Rzeczycki; Rahul K Keswani; Mikhail D Murashov; Zhenzhen Fan; Cheri X Deng; Gus R Rosania
Journal:  Sci Rep       Date:  2019-04-05       Impact factor: 4.379

Review 6.  Biological Effects of Culture Substrates on Human Pluripotent Stem Cells.

Authors:  Yohei Hayashi; Miho Kusuda Furue
Journal:  Stem Cells Int       Date:  2016-08-30       Impact factor: 5.443
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.