Renan O Silva1, Samara R B Damasceno1, Tarcísio V Brito2, Jordana M Dias2, Amanda M Fontenele2, Isabela S Braúna2, José S C Júnior2, Jeanny S Maciel3, Regina C M de Paula3, Ronaldo A Ribeiro1, Marcellus H L P Souza1, Ana L P Freitas4, Jand-Venes R Medeiros2, Draulio C Silva5, André L R Barbosa2. 1. Laboratory of Pharmacology of Inflammation and Cancer, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Ceará, Brazil. 2. Laboratory of Experimental Physiopharmacology, Biotechnology and Biodiversity Center Research (BIOTEC), Federal University of Piauí, Parnaíba, Piauí, Brazil. 3. Laboratory of Polymer, Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, Ceará, Brazil. 4. Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Ceará, Brazil. 5. Laboratory of Biochemistry, Core of Molecular Ecology (NECMOL), Federal University of San Francisco Valley, Petrolina, Pernambuco, Brazil.
Abstract
OBJECTIVES: The aim of the study was to investigate the anti-inflammatory, antioxidant and antinociceptive actions of PFPe, a polysaccharide fraction isolated from the dried fruit of the Passiflora edulis. METHODS: Animals were pretreated with PFPe (0.3, 1 or 3 mg/kg, i.p.) 1 h before induction of paw oedema by carrageenan, histamine, serotonin, compound 48/80 or prostaglandin E2 (PGE2). Neutrophil migration and vascular permeability were measured after carrageenan injection into the peritoneum, and the action of the PFPe on the tumour necrosis factor-alpha, interleukin-1 beta (IL-1β), myeloperoxidase (MPO), glutathione (GSH) and malondialdehyde (MDA) levels was also evaluated. To assay nociception, we examined acetic acid-induced writhing, formalin-induced paw licking and response latency in the hot plate test. KEY FINDINGS: Pretreatment with PFPe significantly inhibited carrageenan-induced paw oedema. PFPe also reduced paw oedema induced by compound 48/80, histamine, serotonin, and PGE2 and compound 48/80-induced vascular permeability. In addition, PFPe significantly reduced the MPO activity, MDA and GSH concentrations, and IL-1β level. In the nociception tests, PFPe reduced acetic acid-induced writhing and formalin-induced paw licking and did not increase the response latency time. CONCLUSIONS: Our results suggest that PFPe administration reduces the inflammatory response by modulation of the liberation or synthesis of histamine and serotonin, by reduction of neutrophil migration, IL-1β levels, and oxidative stress and nociception.
OBJECTIVES: The aim of the study was to investigate the anti-inflammatory, antioxidant and antinociceptive actions of PFPe, a polysaccharide fraction isolated from the dried fruit of the Passiflora edulis. METHODS: Animals were pretreated with PFPe (0.3, 1 or 3 mg/kg, i.p.) 1 h before induction of paw oedema by carrageenan, histamine, serotonin, compound 48/80 or prostaglandin E2 (PGE2). Neutrophil migration and vascular permeability were measured after carrageenan injection into the peritoneum, and the action of the PFPe on the tumour necrosis factor-alpha, interleukin-1 beta (IL-1β), myeloperoxidase (MPO), glutathione (GSH) and malondialdehyde (MDA) levels was also evaluated. To assay nociception, we examined acetic acid-induced writhing, formalin-induced paw licking and response latency in the hot plate test. KEY FINDINGS: Pretreatment with PFPe significantly inhibited carrageenan-induced paw oedema. PFPe also reduced paw oedema induced by compound 48/80, histamine, serotonin, and PGE2 and compound 48/80-induced vascular permeability. In addition, PFPe significantly reduced the MPO activity, MDA and GSH concentrations, and IL-1β level. In the nociception tests, PFPe reduced acetic acid-induced writhing and formalin-induced paw licking and did not increase the response latency time. CONCLUSIONS: Our results suggest that PFPe administration reduces the inflammatory response by modulation of the liberation or synthesis of histamine and serotonin, by reduction of neutrophil migration, IL-1β levels, and oxidative stress and nociception.
Authors: Mariana de Souza Costa; Ramon Handerson Gomes Teles; Yago Medeiros Dutra; José Carlos Rapozo Mazulo Neto; Tarcisio Vieira de Brito; Francisca Francisete de Sousa Nunes Queiroz; Donária Bezerra Nobre do Vale; Luan Kelves Miranda de Souza; Irismara Sousa Silva; André Luiz Dos Reis Barbosa; Jand-Venes Rolim Medeiros; Nivaldo Antonio Parizotto; Marcelo de Carvalho Filgueiras Journal: Lasers Med Sci Date: 2018-06-28 Impact factor: 3.161
Authors: Anamaria Falcão Pereira; Mario Roberto Pontes Lisboa; Bruno Wesley de Freitas Alves; Cristiane Maria Pereira da Silva; Diego Bernarde Souza Dias; Karoline Luanne Santos de Menezes; Francisco Rafael Alves Santana Cesário; Jonas Costa de França; Amanda Rocha de Oliveira; Jaime Eduardo Cecilio Hallak; Antonio Waldo Zuardi; José Alexandre Crippa; Nylane Maria Nunes de Alencar; Roberto César Pereira Lima-Júnior; Mariana Lima Vale Journal: Neurotox Res Date: 2021-11-18 Impact factor: 3.911