BACKGROUND: Colorectal cancer (CRC) is a severe disease with considerable morbidity and mortality. Stable presence of miRNAs in serum enables them to be biomarkers for early diagnosis and surveillance of the progress of CRC. METHODS: The blood samples of 53 CRC patients of I-IV stage pre therapy and 50 controlled healthy people were collected and total serum RNA was extracted. The level of miR-423-5p and miR-484 in serum were detected by qPCR. RESULTS: In stage I-II CRC, serum miR-423-5p was significantly elevated compared with the control, whereas miR-484 was attenuated. In stage III-IV, no significance difference in miR-423-5p level was found between CRC patients and the control, and miR-484 level increased dramatically. CONCLUSIONS: The study provides the possibility that the detection of miR-484-5p and miR-484 may be a useful method for early diagnosis and surveillance of the progress of CRC.
BACKGROUND:Colorectal cancer (CRC) is a severe disease with considerable morbidity and mortality. Stable presence of miRNAs in serum enables them to be biomarkers for early diagnosis and surveillance of the progress of CRC. METHODS: The blood samples of 53 CRC patients of I-IV stage pre therapy and 50 controlled healthy people were collected and total serum RNA was extracted. The level of miR-423-5p and miR-484 in serum were detected by qPCR. RESULTS: In stage I-II CRC, serum miR-423-5p was significantly elevated compared with the control, whereas miR-484 was attenuated. In stage III-IV, no significance difference in miR-423-5p level was found between CRC patients and the control, and miR-484 level increased dramatically. CONCLUSIONS: The study provides the possibility that the detection of miR-484-5p and miR-484 may be a useful method for early diagnosis and surveillance of the progress of CRC.