Correction: Dynamics of actin waves on patterned substrates: a quantitative analysis of circular dorsal ruffles.

Due to a typesetting error, Fig. 2 is missing an essential variable (the Greek τ “tau”) in three places. The publisher apologizes for the error. Please view the complete, correct Fig. 2 here.

Fig 2

Oscillatory reappearing CDRs.

(A) CDRs under spatial confinement exhibit oscillatory patterns of pulsating re-appearance (scale bar: 25 μm, full sequence: S5 Movie). (B) Stills from the region of interest highlighted red in the time-lapse sequence A (Δt = 36 s). (C) A plot of the minimal intensity value of the ROI in A as a function of time shows CDR events as negative peaks and CDR-free periods, corresponding to the recovery time τ, as plateaus of high intensity. The ROI was smoothed with a Gaussian with σ = 2 μm prior to intensity sampling. (D-F) Kymographs of CDRs taken along lines crossing CDR origins (see Fig. 4Afor illustration) show both the recovery time τ between successive events and their radial extension R max (cells not shown). (G) The recovery times increase with CDR size. The data was binned in R max-direction (box width: 10 μm) and plotted as boxes with whiskers (red lines: median, upper box edge: 75th percentile, lower box edge: 25th percentile). N values denote the number of observations. Note that oscillatory behavior was rare for large CDRs.