| Literature DB >> 25806792 |
Yinghan Chen1, Yiming Meng, Yan Cao, Hua Wen, Hong Luo, Xinghua Gao, Fengping Shan.
Abstract
Our understanding of the mechanisms of effect of Ginkgo Seed Polysaccharides (GSPs) on the immune system remains unclear. The aim of this work was to investigate the effect of GSPs on the maturation and function of bone-marrow-derived dendritic cells (BMDCs). The results demonstrate that GSP could exert positive immune modulation on the maturation and functions of BMDCs. This effect was evidenced by decreased changes of phagosome number inside BMDCs, decreased activity of acidic phosphatase (ACP), decreased phagocytosis of BMDCs, and increased changes of key membrane molecules on BMDCs. Upregulated production of cytokines IL-12 and TNF-α also was confirmed. Therefore, it can be concluded that GSPs can efficiently induce the maturation of BMDCs. Our exploration provides direct data and a rationale for potential application of GSPs as an immune enhancer in improving immunity and as a potent adjuvant in the design of DC-based vaccines.Entities:
Keywords: ACP-Acidic phosphatase; APCs-Antigen presenting cells; BMC-Bone marrow cell; BMDCs-Bone marrow derived dendritic cells; DCs-dendritic cells; ELISA-Enzyme linked immunosorbent assay; FCM-Flow cytometry; GM-CSF-Granulocyte macrophage colony stimulating factor; GSPs-Gingko seed polysaccharides; Gingko seed polysaccharides; IL-4-interleukin-4; LPS-Lipopolysaccharide; MACS-Magnetic activated cell sorting; MTS-methyl tolyl sulfide; PBS-Phosphate buffer solution; TEM-Transmission electron microscopy; bone-marrow derived dendritic cells; immune system; maturation; modulation
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Year: 2015 PMID： 25806792 PMCID： PMC4514287 DOI： 10.1080/21645515.2015.1023971
Source DB: PubMed Journal: Hum Vaccin Immunother ISSN： 2164-5515 Impact factor: 3.452