| Literature DB >> 25805002 |
Edinara Targino de Melo1, Andréia Bergamo Estrela1, Elizabeth Cristina Gomes Santos1, Paula Renata Lima Machado2, Kleber Juvenal Silva Farias2, Taffarel Melo Torres3, Enéas Carvalho4, João Paulo Matos Santos Lima5, Arnóbio Antonio Silva-Júnior1, Euzébio Guimarães Barbosa6, Matheus de Freitas Fernandes-Pedrosa7.
Abstract
A new antimicrobial peptide, herein named Stigmurin, was selected based on a transcriptomic analysis of the Brazilian yellow scorpion Tityus stigmurus venom gland, an underexplored source for toxic peptides with possible biotechnological applications. Stigmurin was investigated in silico, by circular dichroism (CD) spectroscopy, and in vitro. The CD spectra suggested that this peptide interacts with membranes, changing its conformation in the presence of an amphipathic environment, with predominance of random coil and beta-sheet structures. Stigmurin exhibited antibacterial and antifungal activity, with minimal inhibitory concentrations ranging from 8.7 to 69.5μM. It was also showed that Stigmurin is toxic against SiHa and Vero E6 cell lines. The results suggest that Stigmurin can be considered a potential anti-infective drug.Entities:
Keywords: Amphipathic peptide; Antimicrobial peptide (AMP); Circular dichroism (CD); Non-disulfide-bridged peptide (NDBP); Scorpion venom; Tityus stigmurus
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Year: 2015 PMID： 25805002 DOI： 10.1016/j.peptides.2015.03.003
Source DB: PubMed Journal: Peptides ISSN： 0196-9781 Impact factor: 3.750