Literature DB >> 25804506

It's a dirty job--A robust method for the purification and de novo genome assembly of Cryptosporidium from clinical material.

Sofia Andersson1, Per Sikora1, Maria L Karlberg1, Jadwiga Winiecka-Krusnell1, Erik Alm1, Jessica Beser1, Romanico B G Arrighi2.   

Abstract

We have developed a novel strategy for the purification of Cryptosporidium oocysts from clinical samples using IMS and PCR amplification of target DNA to facilitate uniform coverage genome sequencing and de novo assembly. Our procedure could also be used for other microbial pathogens from clinical specimens.
Copyright © 2015 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Clinical parasitology; Cryptosporidium; Genome sequencing; Immunomagnetic separation

Mesh:

Substances:

Year:  2015        PMID: 25804506     DOI: 10.1016/j.mimet.2015.03.018

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  6 in total

1.  Genomic Variation in IbA10G2 and Other Patient-Derived Cryptosporidium hominis Subtypes.

Authors:  Per Sikora; Sofia Andersson; Jadwiga Winiecka-Krusnell; Björn Hallström; Cecilia Alsmark; Karin Troell; Jessica Beser; Romanico B G Arrighi
Journal:  J Clin Microbiol       Date:  2016-12-21       Impact factor: 5.948

Review 2.  Comparative genomics: how has it advanced our knowledge of cryptosporidiosis epidemiology?

Authors:  Yingying Fan; Yaoyu Feng; Lihua Xiao
Journal:  Parasitol Res       Date:  2019-11-14       Impact factor: 2.289

Review 3.  Update on Cryptosporidium spp.: highlights from the Seventh International Giardia and Cryptosporidium Conference.

Authors:  Giovanni Widmer; David Carmena; Martin Kváč; Rachel M Chalmers; Jessica C Kissinger; Lihua Xiao; Adam Sateriale; Boris Striepen; Fabrice Laurent; Sonia Lacroix-Lamandé; Gilles Gargala; Loïc Favennec
Journal:  Parasite       Date:  2020-03-13       Impact factor: 3.000

Review 4.  An update on Cryptosporidium biology and therapeutic avenues.

Authors:  Ajit Kumar Dhal; Chinmaya Panda; Soon-Il Yun; Rajani Kanta Mahapatra
Journal:  J Parasit Dis       Date:  2022-06-22

5.  Cryptosporidium as a testbed for single cell genome characterization of unicellular eukaryotes.

Authors:  Karin Troell; Björn Hallström; Anna-Maria Divne; Cecilia Alsmark; Romanico Arrighi; Mikael Huss; Jessica Beser; Stefan Bertilsson
Journal:  BMC Genomics       Date:  2016-06-23       Impact factor: 3.969

Review 6.  Molecular epidemiologic tools for waterborne pathogens Cryptosporidium spp. and Giardia duodenalis.

Authors:  Lihua Xiao; Yaoyu Feng
Journal:  Food Waterborne Parasitol       Date:  2017-09-29
  6 in total

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