AIM: The aim of the study was to assess the therapeutic effect and migration of adoptively transferred Tregs in the course of collagen-induced arthritis (CIA) in rats. METHODS: Sorted CD4(+)CD25(+) cells were cultured in the presence of 17-β-estradiol, stained with CellTracker and then administered into the articular capsule of ankle joint of animals in different stages of CIA progression. RESULTS: Tregs diminished CIA signs only in animals with less advanced disease progress. Moreover, migration of transferred cells into the LN in the near proximity of the injection site and with distal location was almost completely stopped in animals with fully developed CIA. CONCLUSION: Disease progression-related differences in migratory potential of in vitro induced Tregs may be responsible for the failure of cellular therapy during the advanced stages of CIA.
AIM: The aim of the study was to assess the therapeutic effect and migration of adoptively transferred Tregs in the course of collagen-induced arthritis (CIA) in rats. METHODS: Sorted CD4(+)CD25(+) cells were cultured in the presence of 17-β-estradiol, stained with CellTracker and then administered into the articular capsule of ankle joint of animals in different stages of CIA progression. RESULTS:Tregs diminished CIA signs only in animals with less advanced disease progress. Moreover, migration of transferred cells into the LN in the near proximity of the injection site and with distal location was almost completely stopped in animals with fully developed CIA. CONCLUSION: Disease progression-related differences in migratory potential of in vitro induced Tregs may be responsible for the failure of cellular therapy during the advanced stages of CIA.
Entities:
Keywords:
adoptive transfer; collagen-induced arthritis; estrogen; foxp3; regulatory T cells
Authors: Genevieve N Mullins; Kristen M Valentine; Mufadhal Al-Kuhlani; Dan Davini; Kirk D C Jensen; Katrina K Hoyer Journal: Sci Rep Date: 2020-12-15 Impact factor: 4.379