Y Z Igci1, E Bozgeyik1, E Borazan2, E Pala3, A Suner4, M Ulasli1, S A Gurses5, O Yumrutas6, A A Balik2, M Igci1. 1. University of Gaziantep, Faculty of Medicine, Department of Medical Biology, Gaziantep 27310, Turkey. 2. University of Gaziantep, Faculty of Medicine, Department of General Surgery, Gaziantep 27310, Turkey. 3. Sanko University, Faculty of Medicine, Department of Medical Biology, Gaziantep 27090, Turkey. 4. Adiyaman Education and Research Hospital, Adiyaman 02200, Turkey. 5. Zirve University, Faculty of Medicine, Department of Medical Biology, Gaziantep 27260, Turkey. 6. Adiyaman University, Faculty of Medicine, Department of Medical Biology, Adiyaman 02040, Turkey.
Abstract
AIM: The expression differences of SCN8A (which encodes type VIII alpha subunit of voltage gated sodium channel) and NDUFC2 (which encodes C2 subunit of Complex I enzyme in oxidative phosphorylation) genes were evaluated in paired colorectal cancer (CRC) tissues which was relied on our partial transcriptome analysis data in cancer cell lines. MATERIALS AND METHODS: A total of 62 paired tissues of CRC patients (34 male, 28 female) were included in the study. The mRNA levels of SCN8A and NDUFC2 genes were determined by using real-time PCR (qRT-PCR and semiquantitative PCR). RESULTS: SCN8A gene expression level was significantly lower in tumor tissues (p = 0.0128) and in the patients with the age below 45 years (p = 0.0049). There were also meaningful relationships between the gender, grade of CRC, tumor location, histopathological classification, and SCN8A expression. There was no NDUFC2 differential expression. However, the tumors taken from right colon had significantly lower NDUFC2 expression. CONCLUSION: Although the voltage gated sodium channels (VGSCs) and Complex I (CI) were associated to a number of diseases including different types of cancers, the different subunits of CI and individual members of VGSCs seem to be cancer type-specific in varying proportions.
AIM: The expression differences of SCN8A (which encodes type VIII alpha subunit of voltage gated sodium channel) and NDUFC2 (which encodes C2 subunit of Complex I enzyme in oxidative phosphorylation) genes were evaluated in paired colorectal cancer (CRC) tissues which was relied on our partial transcriptome analysis data in cancer cell lines. MATERIALS AND METHODS: A total of 62 paired tissues of CRC patients (34 male, 28 female) were included in the study. The mRNA levels of SCN8A and NDUFC2 genes were determined by using real-time PCR (qRT-PCR and semiquantitative PCR). RESULTS:SCN8A gene expression level was significantly lower in tumor tissues (p = 0.0128) and in the patients with the age below 45 years (p = 0.0049). There were also meaningful relationships between the gender, grade of CRC, tumor location, histopathological classification, and SCN8A expression. There was no NDUFC2 differential expression. However, the tumors taken from right colon had significantly lower NDUFC2 expression. CONCLUSION: Although the voltage gated sodium channels (VGSCs) and Complex I (CI) were associated to a number of diseases including different types of cancers, the different subunits of CI and individual members of VGSCs seem to be cancer type-specific in varying proportions.
Authors: Osbaldo Lopez-Charcas; Piyasuda Pukkanasut; Sadanandan E Velu; William J Brackenbury; Tim G Hales; Pierre Besson; Juan Carlos Gomora; Sébastien Roger Journal: iScience Date: 2021-03-06
Authors: Speranza Rubattu; Sara Di Castro; Herbert Schulz; Aron M Geurts; Maria Cotugno; Franca Bianchi; Henrike Maatz; Oliver Hummel; Samreen Falak; Rosita Stanzione; Simona Marchitti; Stefania Scarpino; Betti Giusti; Ada Kura; Gian Franco Gensini; Flora Peyvandi; Pier Mannuccio Mannucci; Maurizia Rasura; Sebastiano Sciarretta; Melinda R Dwinell; Norbert Hubner; Massimo Volpe Journal: J Am Heart Assoc Date: 2016-02-17 Impact factor: 5.501