Literature DB >> 25801098

Utilization of a reporter system based on the blue pigment indigoidine biosynthetic gene bpsA for detection of promoter activity and deletion of genes in Streptomyces.

Renata Knirschova1, Renata Novakova1, Erik Mingyar1, Carmen Bekeova1, Dagmar Homerova1, Jan Kormanec2.   

Abstract

The integrative promoter-probe plasmid pBPSA1 was constructed using a promoterless Streptomyces aureofaciens CCM3239 bpsA gene encoding a non-ribosomal peptide synthase for the biosynthesis of a blue pigment, indigoidine. bpsA was also used to prepare pAMR4 plasmid for the deletion of genes in Streptomyces with facile identification of double crossover recombination.
Copyright © 2015 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Actinorhodin; Gene disruption; Indigoidine; Promoter-probe vector; Reporter gene; Streptomyces

Mesh：

Substances：

Year:  2015        PMID： 25801098     DOI： 10.1016/j.mimet.2015.03.017

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  3 in total

1.  Secretome Dynamics in a Gram-Positive Bacterial Model.

Authors:  Konstantinos C Tsolis; Mohamed Belal Hamed; Kenneth Simoens; Joachim Koepff; Tobias Busche; Christian Rückert; Marco Oldiges; Jörn Kalinowski; Jozef Anné; Jan Kormanec; Kristel Bernaerts; Spyridoula Karamanou; Anastassios Economou
Journal:  Mol Cell Proteomics       Date:  2018-11-29       Impact factor: 5.911

2.  A sensitive single-enzyme assay system using the non-ribosomal peptide synthetase BpsA for measurement of L-glutamine in biological samples.

Authors:  Alistair S Brown; Katherine J Robins; David F Ackerley
Journal:  Sci Rep       Date:  2017-01-31       Impact factor: 4.379

3.  Marker-Free Genome Engineering in Amycolatopsis Using the pSAM2 Site-Specific Recombination System.

Authors:  Luísa D F Santos; Laëtitia Caraty-Philippe; Emmanuelle Darbon; Jean-Luc Pernodet
Journal:  Microorganisms       Date:  2022-04-16
  3 in total

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