| Literature DB >> 25800778 |
Kazuki Kurimoto1, Yukihiro Yabuta2, Katsuhiko Hayashi3, Hiroshi Ohta2, Hiroshi Kiyonari4, Tadahiro Mitani5, Yoshinobu Moritoki6, Kenjiro Kohri7, Hiroshi Kimura8, Takuya Yamamoto9, Yuki Katou10, Katsuhiko Shirahige10, Mitinori Saitou11.
Abstract
Germ cell specification is accompanied by epigenetic remodeling, the scale and specificity of which are unclear. Here, we quantitatively delineate chromatin dynamics during induction of mouse embryonic stem cells (ESCs) to epiblast-like cells (EpiLCs) and from there into primordial germ cell-like cells (PGCLCs), revealing large-scale reorganization of chromatin signatures including H3K27me3 and H3K9me2 patterns. EpiLCs contain abundant bivalent gene promoters characterized by low H3K27me3, indicating a state primed for differentiation. PGCLCs initially lose H3K4me3 from many bivalent genes but subsequently regain this mark with concomitant upregulation of H3K27me3, particularly at developmental regulatory genes. PGCLCs progressively lose H3K9me2, including at lamina-associated perinuclear heterochromatin, resulting in changes in nuclear architecture. T recruits H3K27ac to activate BLIMP1 and early mesodermal programs during PGCLC specification, which is followed by BLIMP1-mediated repression of a broad range of targets, possibly through recruitment and spreading of H3K27me3. These findings provide a foundation for reconstructing regulatory networks of the germline epigenome.Entities:
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Year: 2015 PMID： 25800778 DOI： 10.1016/j.stem.2015.03.002
Source DB: PubMed Journal: Cell Stem Cell ISSN： 1875-9777 Impact factor: 24.633