Correction: Increased N-glycosylation efficiency by generation of an aromatic sequon on N135 of antithrombin.

The images for Figs. 3 and 4 are incorrectly switched. The image that appears as Fig. 3 should be Fig. 4, and the image that appears as Fig. 4 should be Fig. 3. The figure legends appear in the correct order.

Fig 3

Function of antithrombin variants.

Anti-FXa activity of antithrombin proteins secreted to the conditioned medium in presence of heparin. Results are expressed as a percentage of the activity of the S137T variant. Each bar represents the mean ± standard deviation (SD) of two independent experiments performed in duplicate. The differences between mutants were tested by paired t-test (p-value). The “*” indicated differences statistically significant with p<0.05.

Fig 4

Scheme of binding of antithrombin and heparin.

Initial rapid equilibrium, K , between antithrombin, AT, and pentasaccharide, H, leads to complex, AT.H, followed by rapid conformational change via k to a high heparin affinity, highly fluorescence complex, AT*.H.

There are errors in the Van't Hoff equation under the Materials and Methods subsection titled “Determination of denaturing temperature.” Please view the complete, correct equation here: