| Literature DB >> 25799188 |
.
Abstract
Entities:
Year: 2015 PMID: 25799188 PMCID: PMC4370628 DOI: 10.1371/journal.pone.0119399
Source DB: PubMed Journal: PLoS One ISSN: 1932-6203 Impact factor: 3.240
Fig 2NO modulation of glutamate responses in type 4 CBCs.
(A) Representative recordings of glutamate responses of a type 4 OFF CBC, clamped to –60 mV. The experimental setup (A1) and an image of the lucifer yellow-filled recorded cell (A2) are shown to the left. (A3) Application of NO donor NOC-12 (200 μM) only affected the slow component of the glutamate response, by shortening the duration of the electrical response. Bars indicate the stimulus duration. (A4) Bar diagrams displaying the mean ± SEM of the total charge transferred during the glutamate response, with and without NO stimulation. (A5) The maximum amplitude of the glutamate response, measured at the peak of the fast component, remained unaffected by NO. (B) Control experiments with puffs of extracellular solution instead of NOC-12 were ineffective, demonstrating the absence of stimulus or pressure artifacts. (C) Bath application of the GABAA and GABAC receptor antagonists SR-95531 and TPMPA, and the glycine receptor blocker strychnine did not affect the modulation of the glutamate response by NO in type 4 CBCs. Image scale bars = 10 μm; ns = not significant.