| Literature DB >> 25799062 |
Yee Han Tee1, Tom Shemesh2, Visalatchi Thiagarajan1, Rizal Fajar Hariadi3, Karen L Anderson4, Christopher Page4, Niels Volkmann4, Dorit Hanein4, Sivaraj Sivaramakrishnan3, Michael M Kozlov5, Alexander D Bershadsky6.
Abstract
Cellular mechanisms underlying the development of left-right asymmetry in tissues and embryos remain obscure. Here, the development of a chiral pattern of actomyosin was revealed by studying actin cytoskeleton self-organization in cells with isotropic circular shape. A radially symmetrical system of actin bundles consisting of α-actinin-enriched radial fibres (RFs) and myosin-IIA-enriched transverse fibres (TFs) evolved spontaneously into the chiral system as a result of the unidirectional tilting of all RFs, which was accompanied by a tangential shift in the retrograde movement of TFs. We showed that myosin-IIA-dependent contractile stresses within TFs drive their movement along RFs, which grow centripetally in a formin-dependent fashion. The handedness of the chiral pattern was shown to be regulated by α-actinin-1. Computational modelling demonstrated that the dynamics of the RF-TF system can explain the pattern transition from radial to chiral. Thus, actin cytoskeleton self-organization provides built-in machinery that potentially allows cells to develop left-right asymmetry.Entities:
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Year: 2015 PMID： 25799062 DOI： 10.1038/ncb3137
Source DB: PubMed Journal: Nat Cell Biol ISSN： 1465-7392 Impact factor: 28.824