Peng-Ling Li1, Hong-Guo Sun1, Yong-Li Hua1, Peng Ji1, Ling Zhang1, Jin-Xia Li1, Yanming Wei2. 1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China. 2. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China. Electronic address: weiym@gsau.edu.cn.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Angelica sinensis (AS) has been used in traditional Chinese medicine for thousands of years to enrich and invigorate blood. In this study, the aim is to investigate the influence of AS on metabolism of blood deficiency mice model and to explore its anti-blood deficiency mechanism. MATERIALS AND METHODS: The blood deficiency mice model was induced by being hypodermically injected with N-acetyl phenylhydrazine (APH) and being intraperitoneally injected with cyclophosphamide (CTX). Gas chromatography-mass spectrometry (GC-MS), principle component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used to identify potential biomarkers in plasma and splenic tissue. RESULTS: The levels of white blood cell (WBC), red blood cell (RBC), hemoglobin (HGB) and platelet (PLT) showed a trend to return to control group after administrating with AS, while the dose of 10g/kg showed the best effect. Potential metabolite biomarkers (nine in the plasma and nine in the spleen homogenates) were identified in this study. These biomarkers were mainly related to five metabolic pathways, such as arachidonic acid metabolism, valine, leucine and isoleucine biosynthesis, glycine, serine and threonine metabolism, arginine and proline metabolism and TCA cycle. CONCLUSION: Metabolomics was used to reflect an organism׳s physiological and metabolic state comprehensively, indicating that metabolomics was a potentially powerful tool to reveal the anti-blood deficiency mechanism of AS.
ETHNOPHARMACOLOGICAL RELEVANCE: Angelica sinensis (AS) has been used in traditional Chinese medicine for thousands of years to enrich and invigorate blood. In this study, the aim is to investigate the influence of AS on metabolism of blood deficiencymice model and to explore its anti-blood deficiency mechanism. MATERIALS AND METHODS: The blood deficiencymice model was induced by being hypodermically injected with N-acetyl phenylhydrazine (APH) and being intraperitoneally injected with cyclophosphamide (CTX). Gas chromatography-mass spectrometry (GC-MS), principle component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used to identify potential biomarkers in plasma and splenic tissue. RESULTS: The levels of white blood cell (WBC), red blood cell (RBC), hemoglobin (HGB) and platelet (PLT) showed a trend to return to control group after administrating with AS, while the dose of 10g/kg showed the best effect. Potential metabolite biomarkers (nine in the plasma and nine in the spleen homogenates) were identified in this study. These biomarkers were mainly related to five metabolic pathways, such asarachidonic acid metabolism, valine, leucine and isoleucine biosynthesis, glycine, serine and threonine metabolism, arginine and proline metabolism and TCA cycle. CONCLUSION: Metabolomics was used to reflect an organism׳s physiological and metabolic state comprehensively, indicating that metabolomics was a potentially powerful tool to reveal the anti-blood deficiency mechanism of AS.
Authors: Dan He; Wan Dan; Qing Du; Bing-Bing Shen; Lin Chen; Liang-Zi Fang; Jian-Jun Kuang; Chun-Yu Tang; Ping Cai; Rong Yu; Shui-Han Zhang; Jian-Hua Huang Journal: Drug Des Devel Ther Date: 2022-04-28 Impact factor: 4.319
Authors: Kaixue Zhang; Menglin Yan; Shu Han; Longfei Cong; Liyao Wang; Liu Zhang; Lili Sun; Haiying Bai; Guanhua Wei; Hong Du; Min Jiang; Gang Bai; Zhigang Yang Journal: Molecules Date: 2019-09-30 Impact factor: 4.411