Sonia Etenna Lekana-Douki1, Claudine Kombila-Koumavor2, Dieudonné Nkoghe3, Christian Drosten4, Jan Felix Drexler5, Eric M Leroy6. 1. Centre International de Recherches Médicales de Franceville, BP 769 Franceville, Gabon. Electronic address: s_lekana@yahoo.fr. 2. Centre International de Recherches Médicales de Franceville, BP 769 Franceville, Gabon. Electronic address: kombilaalexane@yahoo.fr. 3. Centre International de Recherches Médicales de Franceville, BP 769 Franceville, Gabon; Ministère de la Santé Publique, BP 5978 Libreville, Gabon. Electronic address: dnkoghe@hotmail.com. 4. Institute of Virology, Bonn Medical Centre, Bonn, Germany. Electronic address: drosten@virology-bonn.de. 5. Institute of Virology, Bonn Medical Centre, Bonn, Germany. Electronic address: drexler@virology-bonn.de. 6. Centre International de Recherches Médicales de Franceville, BP 769 Franceville, Gabon; UMR (IRD 224 /CNRS 5290 / UM1-UM2), Institut de Recherche pour le Développement, Montpellier, France. Electronic address: eric.leroy@ird.fr.
Abstract
OBJECTIVES: This study aimed to determine the prevalence of enteric viruses causing gastroenteritis, and the circulating stains, in Gabonese children under five years old who visited health centers between March 2010 and June 2011. METHODS: Stool specimens were collected and sent for analysis to CIRMF (Centre International de Recherches Médicales de Franceville). Stools were screened for six enteric viruses (rotavirus, adenovirus, norovirus I and II, sapovirus, human astrovirus) by means of a multiplex real-time reverse transcription polymerase chain reaction, and Rotavirus A, Adenovirus and Astrovirus were genotyped. RESULTS: Among the 317 specimens analyzed, 193 (60.9%) were positive for at least one enteric virus. Rotavirus A (RVA) (27.1%) was the most frequently detected virus, followed by human Adenovirus (HAdV) (19.6%), Norovirus II (NoVs-II) (13.9%), Norovirus I (NoVs-I) (9.1%), Sapovirus (SaV) (9.5%) and human Astrovirus (HAstV) (6.3%). One-third of the 193 positive samples contained more than one virus. The most common Rotavirus A genotype was G6P[6]. Various HAdV serotypes were found. HAstV-1 was identified. CONCLUSIONS: These findings improve our knowledge of circulating enteric viruses in Gabon. The emergence of unusual G6P[6] strain of rotavirus A, predominant, suggested a particular epidemiological surveillance of circulating rotavirus strains during the introduction of vaccination in Gabon.
OBJECTIVES: This study aimed to determine the prevalence of enteric viruses causing gastroenteritis, and the circulating stains, in Gabonese children under five years old who visited health centers between March 2010 and June 2011. METHODS: Stool specimens were collected and sent for analysis to CIRMF (Centre International de Recherches Médicales de Franceville). Stools were screened for six enteric viruses (rotavirus, adenovirus, norovirus I and II, sapovirus, human astrovirus) by means of a multiplex real-time reverse transcription polymerase chain reaction, and Rotavirus A, Adenovirus and Astrovirus were genotyped. RESULTS: Among the 317 specimens analyzed, 193 (60.9%) were positive for at least one enteric virus. Rotavirus A (RVA) (27.1%) was the most frequently detected virus, followed by human Adenovirus (HAdV) (19.6%), Norovirus II (NoVs-II) (13.9%), Norovirus I (NoVs-I) (9.1%), Sapovirus (SaV) (9.5%) and human Astrovirus (HAstV) (6.3%). One-third of the 193 positive samples contained more than one virus. The most common Rotavirus A genotype was G6P[6]. Various HAdV serotypes were found. HAstV-1 was identified. CONCLUSIONS: These findings improve our knowledge of circulating enteric viruses in Gabon. The emergence of unusual G6P[6] strain of rotavirus A, predominant, suggested a particular epidemiological surveillance of circulating rotavirus strains during the introduction of vaccination in Gabon.
Authors: G La Rosa; S Della Libera; S Petricca; M Iaconelli; D Donia; P Saccucci; F Cenko; G Xhelilaj; M Divizia Journal: Biomed Res Int Date: 2015-08-03 Impact factor: 3.411
Authors: Silvana Augusta Rodrigues Portes; Eduardo de Mello Volotão; Monica Simões Rocha; Maria Cristina Rebelo; Maria da Penha Trindade Pinheiro Xavier; Rosane Maria de Assis; Tatiana Lundgren Rose; Marize Pereira Miagostovich; José Paulo Gagliardi Leite; Filipe Anibal Carvalho-Costa Journal: Mem Inst Oswaldo Cruz Date: 2016-05-24 Impact factor: 2.743