Literature DB >> 2579542

A method for the covalent attachment of cells to glass slides for use in immunohistochemical assays.

J A Maples.   

Abstract

A method for covalently attaching viable cells to glass slides is described. The cells are linked covalently to the slide without the use of drying or other fixation procedures that normally destroy cellular surface antigens. The attachment is permanent, and the slides with attached cells may be cryopreserved. The use of the avidin-biotin-peroxidase procedure with this procedure shows excellent sensitivity comparable to flow cytometry.

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Year:  1985        PMID: 2579542     DOI: 10.1093/ajcp/83.3.356

Source DB:  PubMed          Journal:  Am J Clin Pathol        ISSN: 0002-9173            Impact factor:   2.493


  21 in total

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Authors:  B Slobedman; E S Mocarski
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2.  Mast cell granule heparin proteoglycan induces lacunae in confluent endothelial cell monolayers.

Authors:  D Lagunoff; A Rickard
Journal:  Am J Pathol       Date:  1999-05       Impact factor: 4.307

3.  Scrapie-associated prion protein accumulates in astrocytes during scrapie infection.

Authors:  J F Diedrich; P E Bendheim; Y S Kim; R I Carp; A T Haase
Journal:  Proc Natl Acad Sci U S A       Date:  1991-01-15       Impact factor: 11.205

4.  In situ hybridisation in herpetic lesions using a biotinylated DNA probe.

Authors:  M Dictor; E Renfjärd; A Brun
Journal:  J Clin Pathol       Date:  1990-05       Impact factor: 3.411

5.  Viruses and interstitial cystitis: adenovirus genomes cannot be demonstrated in urinary bladder biopsies.

Authors:  V Hukkanen; M Haarala; M Nurmi; P Klemi; P Kiilholma
Journal:  Urol Res       Date:  1996

6.  Human immunodeficiency virus type 1 in spinal cords of acquired immunodeficiency syndrome patients with myelopathy: expression and replication in macrophages.

Authors:  D J Eilbott; N Peress; H Burger; D LaNeve; J Orenstein; H E Gendelman; R Seidman; B Weiser
Journal:  Proc Natl Acad Sci U S A       Date:  1989-05       Impact factor: 11.205

7.  Quest for a reliable, valid, and sensitive in situ hybridization procedure to detect viral nucleic acids in the central nervous system.

Authors:  W W Tourtellotte; P Schmid; P Pick; N Verity; S Martinez; P Shapshak
Journal:  Neurochem Res       Date:  1987-06       Impact factor: 3.996

8.  Glass slide models for immunocytochemistry and in situ hybridization.

Authors:  L I Larsson; D M Hougaard
Journal:  Histochemistry       Date:  1994-06

9.  Expression of the L2 and E7 genes of the human papillomavirus type 16 in female genital dysplasias.

Authors:  E Auvinen; H Kujari; P Arstila; V Hukkanen
Journal:  Am J Pathol       Date:  1992-11       Impact factor: 4.307

10.  Replication of rat virus in neonatal calvaria in culture.

Authors:  G S Schuster; N L O'Dell; J T Wilson; D C Ward
Journal:  In Vitro Cell Dev Biol       Date:  1988-10
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