Literature DB >> 2579477

A widely applicable analytical system for biological stains: reverse-phase thin layer chromatography.

G B Proctor, R W Horobin.   

Abstract

The suitability of reverse-phase thin layer chromatography using a commercial adsorbant and aqueous methanol as an analytical tool for biological stains was investigated. The wide range of applicability of this technique is shown by the fact that of 120 dyes used as biological stains, 84 of diverse chemical character were successfully chromatographed by varying only the water content of the eluent. Unsuccessful chromatography was due either to immobility or streaking. Dyes exhibiting this behavior can be identified prior to chromatography by inspection of their structural formulae. Rf values were found to be significantly correlated with the calculated partition coefficients. This relationship provides information for the identification of dye components revealed by chromatography and a discussion of its use in the chemical characterization of various dyestuffs is presented.

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Year:  1985        PMID： 2579477     DOI： 10.3109/10520298509113884

Source DB:  PubMed          Journal:  Stain Technol        ISSN： 0038-9153


  8 in total

1.  Dye standards, Part II. 6: Rosaniline (CI 42510). European Committee for Clinical Laboratory Standards (ECCLS), Subcommittee on Reference Materials for Tissue Stains (SRMTS).

Authors: 
Journal:  Histochem J       Date:  1992-04

2.  Dye standards, Part II. 5: Pararosaniline (CI 42500). European Committee for Clinical Laboratory Standards (ECCLS), Subcommittee on Reference Materials for Tissue Stains (SRMTS).

Authors: 
Journal:  Histochem J       Date:  1992-04

3.  Dye standards, Part II. 8: New fuchsin (CI 42520). European Committee for Clinical Laboratory Standards (ECCLS), Subcommittee on Reference Materials for Tissue Stains (SRMTS).

Authors: 
Journal:  Histochem J       Date:  1992-04

4.  Dye standards, Part II. 7: Magenta II (no CI number). European Committee for Clinical Laboratory Standards (ECCLS), Subcommittee on Reference Materials for Tissue Stains (SRMTS).

Authors: 
Journal:  Histochem J       Date:  1992-04

5.  Interaction of molecular probes with living cells and tissues. Part 2. A structure-activity analysis of mitochondrial staining by cationic probes, and a discussion of the synergistic nature of image-based and biochemical approaches.

Authors:  F Rashid; R W Horobin
Journal:  Histochemistry       Date:  1990

6.  Understanding Romanowsky staining. 2. The staining mechanism of suspension-fixed cells, including influences of specimen morphology on the Romanowsky-Giemsa effect.

Authors:  R W Horobin; D Curtis; L Pindar
Journal:  Histochemistry       Date:  1989

7.  Prediction of in situ fluorescence of histochemical reagents using a structure-staining correlation procedure.

Authors:  A Juarranz; R W Horobin; G B Proctor
Journal:  Histochemistry       Date:  1986

8.  Understanding Romanowsky staining. I: The Romanowsky-Giemsa effect in blood smears.

Authors:  R W Horobin; K J Walter
Journal:  Histochemistry       Date:  1987
  8 in total

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