| Literature DB >> 25794733 |
Anna Vallverdú-Queralt1, Nuria Boix2, Ester Piqué3, Jesús Gómez-Catalan4, Alexander Medina-Remon5, Gemma Sasot6, Mercè Mercader-Martí7, Juan M Llobet8, Rosa M Lamuela-Raventos9.
Abstract
The zebrafish embryo is a highly interesting biological model with applications in different scientific fields, such as biomedicine, pharmacology and toxicology. In this study, we used liquid chromatography/electrospray ionisation-linear ion trap quadrupole-Orbitrap-mass spectrometry (HPLC/ESI-LTQ-Orbitrap-MS) to identify the polyphenol compounds in a red wine extract and zebrafish embryos. Phenolic compounds and anthocyanin metabolites were determined in zebrafish embryos previously exposed to the red wine extract. Compounds were identified by injection in a high-resolution system (LTQ-Orbitrap) using accurate mass measurements in MS, MS(2) and MS(3) modes. To our knowledge, this research constitutes the first comprehensive identification of phenolic compounds in zebrafish by HPLC coupled to high-resolution mass spectrometry.Entities:
Keywords: Identification; Orbitrap-MS; Polyphenols; Red wine extract; Zebrafish (ZF)
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Year: 2015 PMID： 25794733 DOI： 10.1016/j.foodchem.2015.02.098
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514