Chiung-Chi Cheng1, Yi-Hsiang Liu2, Yen-Chang Clark Lai3, Yung-Hsiang Hsu4, Wei-Ting Chao5, Yih-Shyong Lai6. 1. Department of Pathology, Chang Bing Show Chwan Memorial Hospital, Changhua County, Taiwan, R.O.C. Center for General Education, Providence University, Taichung City, Taiwan, R.O.C. 2. Department of Pathology, Chang Bing Show Chwan Memorial Hospital, Changhua County, Taiwan, R.O.C. Department of Pathology, Tzu Chi University, Hualien County, Taiwan, R.O.C. 3. Department of Pathology, Kaohsiung Medical University Hospital, Kaohsiung City, Taiwan, R.O.C. 4. Department of Pathology, Tzu Chi University, Hualien County, Taiwan, R.O.C. 5. Department of Life Science, Tunghai University, Taichung City, Taiwan, R.O.C. 6. Department of Pathology, Chang Bing Show Chwan Memorial Hospital, Changhua County, Taiwan, R.O.C. yslaick18@yahoo.com.tw.
Abstract
BACKGROUND: Aberrant histone deacetylase expression may cause imbalance between acetylation and deacetylation of histone and play roles in tumor transformation. We found that histone 3 was modulated in human hepatocellular carcinoma. We determined if histone 3 modulation is related to the aberrant expression of histone deacetylase. MATERIALS AND METHODS: We analyzed human liver and hepatocellular carcinoma tissues and fibroblast and fibrosarcoma cell lines for the expression of histone 3, histone deacetylase 1 and acetylated histone 3 using immunohistochemistry, western blot and immunofluorescence. RESULTS: Histone deacetylase 1 and histone 3 were more strongly detected in hepatocellular carcinoma tissue and fibrosarcoma cells than in liver tissues and fibroblast cells, respectively. However, acetylated histone 3 was more strongly expressed in normal liver and fibroblast cells and less expressed in hepatocellular carcinoma and fibrosarcoma cells. CONCLUSION: Histone deacetylase 1 overexpression and hypoacetylation of histone 3 might play critical roles in the modulation of histone 3 in human hepatocellular carcinoma.
BACKGROUND: Aberrant histone deacetylase expression may cause imbalance between acetylation and deacetylation of histone and play roles in tumor transformation. We found that histone 3 was modulated in humanhepatocellular carcinoma. We determined if histone 3 modulation is related to the aberrant expression of histone deacetylase. MATERIALS AND METHODS: We analyzed human liver and hepatocellular carcinoma tissues and fibroblast and fibrosarcoma cell lines for the expression of histone 3, histone deacetylase 1 and acetylated histone 3 using immunohistochemistry, western blot and immunofluorescence. RESULTS:Histone deacetylase 1 and histone 3 were more strongly detected in hepatocellular carcinoma tissue and fibrosarcoma cells than in liver tissues and fibroblast cells, respectively. However, acetylated histone 3 was more strongly expressed in normal liver and fibroblast cells and less expressed in hepatocellular carcinoma and fibrosarcoma cells. CONCLUSION:Histone deacetylase 1 overexpression and hypoacetylation of histone 3 might play critical roles in the modulation of histone 3 in humanhepatocellular carcinoma.