Emre Sivrikoz1, Özlem Timirci-Kahraman2, Arzu Ergen2, Ümit Zeybek2, Murat Aksoy3, Fatih Yanar4, Turgay İsbir5, Mehmet Kurtoğlu6. 1. Gaziosmanpasa Taksim Training and Research Hospital, Department of Surgery, Istanbul, Turkey. 2. Istanbul University, Institute of Experimental Medicine, Department of Molecular Medicine, Istanbul, Turkey. 3. Liv Hospital, Bahcesehir University, Department of Surgery, Istanbul, Turkey. 4. Istanbul School of Medicine, Istanbul University Department of Surgery, Istanbul, Turkey. 5. Yeditepe University, Faculty of Medicine, Department of Medical Biology, Istanbul, Turkey. 6. Istanbul School of Medicine, Istanbul University Department of Surgery, Istanbul, Turkey metlevkurt@superonline.com.
Abstract
AIM: The purpose of this study was to investigate the gene expression levels of elastin and fibulin-5 according to differences between carotid plaque regions and to correlate it with clinical features of plaque destabilization. MATERIALS AND METHODS: The study included 44 endarterectomy specimens available from operated symptomatic carotid artery stenoses. The specimens were separated according to anatomic location: internal carotid artery (ICA), external carotid artery (ECA) and common carotid artery (CCA), and then stored in liquid nitrogen. The amounts of cDNA for elastin and fibulin-5 were determined by Quantitative real-time PCR (Q-RT-PCR). Target gene copy numbers were normalized using hypoxanthine-guanine phosphoribosyltransferase (HPRT1) gene. The delta-delta CT method was applied for relative quantification. RESULTS: Q-RT-PCR data showed that relative fibulin-5 gene expression was increased in ICA plaque regions when compared to CCA regions but not reaching significance (p=0.061). At the same time, no differences were observed in elastin mRNA level between different anatomic plaque regions (p>0.05). Moreover, elastin and fibulin-5 mRNA expression and clinical parameters were compared in ICA plaques versus CCA and ECA regions, respectively. Up-regulation of elastin and fibulin-5 mRNA levels in ICA were strongly correlated with family history of cardiovascular disease when compared to CCA (p<0.05). Up-regulation of fibulin-5 in ICA was significantly associated with diabetes, and elevated triglycerides and very low density lipoprotein (VLDL) when compared to ECA (p<0.05). CONCLUSION: The clinical significance is the differences between the proximal and distal regions of the lesion, associated with the ICA, CCA and ECA respectively, with increased fibulin-5 in the ICA region.
AIM: The purpose of this study was to investigate the gene expression levels of elastin and fibulin-5 according to differences between carotid plaque regions and to correlate it with clinical features of plaque destabilization. MATERIALS AND METHODS: The study included 44 endarterectomy specimens available from operated symptomatic carotid artery stenoses. The specimens were separated according to anatomic location: internal carotid artery (ICA), external carotid artery (ECA) and common carotid artery (CCA), and then stored in liquid nitrogen. The amounts of cDNA for elastin and fibulin-5 were determined by Quantitative real-time PCR (Q-RT-PCR). Target gene copy numbers were normalized using hypoxanthine-guanine phosphoribosyltransferase (HPRT1) gene. The delta-delta CT method was applied for relative quantification. RESULTS: Q-RT-PCR data showed that relative fibulin-5 gene expression was increased in ICA plaque regions when compared to CCA regions but not reaching significance (p=0.061). At the same time, no differences were observed in elastin mRNA level between different anatomic plaque regions (p>0.05). Moreover, elastin and fibulin-5 mRNA expression and clinical parameters were compared in ICA plaques versus CCA and ECA regions, respectively. Up-regulation of elastin and fibulin-5 mRNA levels in ICA were strongly correlated with family history of cardiovascular disease when compared to CCA (p<0.05). Up-regulation of fibulin-5 in ICA was significantly associated with diabetes, and elevated triglycerides and very low density lipoprotein (VLDL) when compared to ECA (p<0.05). CONCLUSION: The clinical significance is the differences between the proximal and distal regions of the lesion, associated with the ICA, CCA and ECA respectively, with increased fibulin-5 in the ICA region.
Authors: Maryam Karimi; Marta T Ignasiak; Bun Chan; Anna K Croft; Leo Radom; Carl H Schiesser; David I Pattison; Michael J Davies Journal: Sci Rep Date: 2016-12-12 Impact factor: 4.379
Authors: Claudia C Preston; Tricia D Larsen; Julie A Eclov; Eli J Louwagie; Tyler C T Gandy; Randolph S Faustino; Michelle L Baack Journal: Front Endocrinol (Lausanne) Date: 2020-09-17 Impact factor: 5.555