| Literature DB >> 2579158 |
H Sidberry, B Kaufman, D C Wright, J Sadoff.
Abstract
A rapid, sensitive immunoenzymatic technique for the analysis of lipopolysaccharide (LPS) from gram-negative bacteria using monoclonal antibodies is described. After separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the LPS was either stained with silver or electrophoretically transferred to nitrocellulose. After reaction with anti-LPS monoclonal antibodies, the transferred antigens were visualized by reaction with alkaline-phosphatase-labelled anti-mouse antibodies and a substrate containing naphthol phosphoric acid and Fast Red.Entities:
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Year: 1985 PMID： 2579158 DOI： 10.1016/0022-1759(85)90307-2
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303