Ilona Polur1, Yosuke Kamiya2, Manshan Xu3, Bianca S Cabri4, Marwa Alshabeeb5, Sunil Wadhwa6, Jing Chen7. 1. Division of Orthodontics, Columbia University College of Dental Medicine, New York, NY 10032, USA. Electronic address: ip2162@columbia.edu. 2. Division of Orthodontics, Columbia University College of Dental Medicine, New York, NY 10032, USA. Electronic address: kamisuke816@gmail.com. 3. Division of Orthodontics, Columbia University College of Dental Medicine, New York, NY 10032, USA. Electronic address: mx2137@cumc.columbia.edu. 4. Columbia University College of Dental Medicine, New York, NY 10032, USA. Electronic address: bsc2126@cumc.columbia.edu. 5. Division of Orthodontics, Columbia University College of Dental Medicine, New York, NY 10032, USA. Electronic address: ma3315@columbia.edu. 6. Division of Orthodontics, Columbia University College of Dental Medicine, New York, NY 10032, USA. Electronic address: sw2680@cumc.columbia.edu. 7. Division of Orthodontics, Columbia University College of Dental Medicine, New York, NY 10032, USA. Electronic address: jc3835@cumc.columbia.edu.
Abstract
OBJECTIVE: Temporomandibular joint (TMJ) disorders predominantly afflict women, suggesting that estrogen may play a role in the disease process. Defects in mechanical loading-induced TMJ remodelling are believed to be a major etiological factor in TMJ degenerative disease. Previously, we found that, decreased occlusal loading caused a significant decrease in early chondrocyte maturation markers (Sox9 and Col 2) in female, but not male, C57BL/6 wild type mice (1). The goal of this study was to examine the role of Estrogen Receptor (ER) beta in mediating these effects. DESIGN: 21-day-old male (n = 24) and female (n = 25) ER beta KO mice were exposed to decreased occlusal loading (soft diet administration and incisor trimming) for 4 weeks. At 49 days of age the mice were sacrificed. Proliferation, gene expression, Col 2 immunohistochemistry and micro-CT analysis were performed on the mandibular condyles. RESULTS: Decreased occlusal loading triggered similar effects in male and female ER beta KO mice; specifically, significant decreases in Col 10 expression, subchondral total volume, bone volume, and trabecular number. CONCLUSION: Decreased occlusal loading induced inhibition of chondrocyte maturation markers (Sox9 and Col 2) did not occur in female ER beta deficient mice.
OBJECTIVE: Temporomandibular joint (TMJ) disorders predominantly afflict women, suggesting that estrogen may play a role in the disease process. Defects in mechanical loading-induced TMJ remodelling are believed to be a major etiological factor in TMJ degenerative disease. Previously, we found that, decreased occlusal loading caused a significant decrease in early chondrocyte maturation markers (Sox9 and Col 2) in female, but not male, C57BL/6 wild type mice (1). The goal of this study was to examine the role of Estrogen Receptor (ER) beta in mediating these effects. DESIGN: 21-day-old male (n = 24) and female (n = 25) ER beta KO mice were exposed to decreased occlusal loading (soft diet administration and incisor trimming) for 4 weeks. At 49 days of age the mice were sacrificed. Proliferation, gene expression, Col 2 immunohistochemistry and micro-CT analysis were performed on the mandibular condyles. RESULTS: Decreased occlusal loading triggered similar effects in male and female ER beta KO mice; specifically, significant decreases in Col 10 expression, subchondral total volume, bone volume, and trabecular number. CONCLUSION: Decreased occlusal loading induced inhibition of chondrocyte maturation markers (Sox9 and Col 2) did not occur in female ER beta deficient mice.
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