Nasrin Sohrabi1, Mahmoud Shekari Khaniani2, Sima Mansoori Derakhshan3. 1. Medical Genetic Department, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. ; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. ; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran. 2. Medical Genetic Department, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. 3. Medical Genetic Department, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. ; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract
PURPOSE: Association between HLA-DR4-DQ8 haplotype and type 1 Diabetes Mellitus (DM-1A) was investigated in children of East Azerbaijan state of Iran because such an association has not been previously studied in this population. METHODS: HLA-typing was performed by polymerase chain reaction sequence-specific priming. For haplotype analysis, the logistic regression model was performed. RESULTS: Of the three investigated alleles, the frequency of DRB1*0401 was significantly higher among patients compared with that in healthy subjects (76.74% vs. 23.26%). CONCLUSION: The findings of the current study are consistent with those of previous studies and show that DRB1*0401 is associated with DM-1A; the frequencies of the two other alleles were also higher among patients, although the differences were not statistically significant. Two haplotypes associated with these alleles were also surveyed, and DRB1*0401--DQA1*0301-, and DRB1*0401--DQA1*0301--DQB1*0302- were the most frequent haplotypes among the patient group.
PURPOSE: Association between HLA-DR4-DQ8 haplotype and type 1 Diabetes Mellitus (DM-1A) was investigated in children of East Azerbaijan state of Iran because such an association has not been previously studied in this population. METHODS: HLA-typing was performed by polymerase chain reaction sequence-specific priming. For haplotype analysis, the logistic regression model was performed. RESULTS: Of the three investigated alleles, the frequency of DRB1*0401 was significantly higher among patients compared with that in healthy subjects (76.74% vs. 23.26%). CONCLUSION: The findings of the current study are consistent with those of previous studies and show that DRB1*0401 is associated with DM-1A; the frequencies of the two other alleles were also higher among patients, although the differences were not statistically significant. Two haplotypes associated with these alleles were also surveyed, and DRB1*0401--DQA1*0301-, and DRB1*0401--DQA1*0301--DQB1*0302- were the most frequent haplotypes among the patient group.
Entities:
Keywords:
Genotype; HLA-DQ; HLA-DR; Haplotype; Type 1 diabetes
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