Tadiboyina Sirisha1, Bannimath Gurupadayya1, Sridhar Siddiraju2. 1. Department of Pharmaceutical Chemistry, JSS College of Pharmacy, JSS University, Mysore, India. 2. Department of Pharmaceutical Chemistry, Malla Reddy College of Pharmacy, Osmania University, Secunderabad, India.
Abstract
PURPOSE: A validated ultra performance liquid chromatography (UPLC) method has been developed and validated for the simultaneous determination of losartan potassium and chlorthalidone in pharmaceutical preparations. METHODS: Waters-Acquity UPLC system equipped with Auto Sampler, PDA detector and operated with Empower-2 software was used for the present study. Detection was done at wavelength of 230 nm, HSS C18, 100 mm x 2.1x 1.8 μm column with a reverse phase elution and mobile phase composed of A and B mixed in the ratio 56:44 v/v (Where mobile phase A consists of potassium dihydrogen phosphate buffer of pH 3.0 and Mobile phase B consists of acetonitrile and methanol mixed in the ratio of 90:10 v/v) used at a flow rate of 0.4ml per minute. RESULTS: The retention times for losartan potassium and chlorthalidone were observed at 0.72 and 1.89 minutes. The developed method was validated as per ICH guidelines. Linearity ranges were found to be 12.5-125 μg/ml and 3.125-31.25 μg/ml for losartan potassium and chlorthalidone, respectively. CONCLUSION: This method is fast, accurate, precise and sensitive hence it can be employed for routine quality control of tablets containing both drugs in industries.
PURPOSE: A validated ultra performance liquid chromatography (UPLC) method has been developed and validated for the simultaneous determination of losartan potassium and chlorthalidone in pharmaceutical preparations. METHODS: Waters-Acquity UPLC system equipped with Auto Sampler, PDA detector and operated with Empower-2 software was used for the present study. Detection was done at wavelength of 230 nm, HSS C18, 100 mm x 2.1x 1.8 μm column with a reverse phase elution and mobile phase composed of A and B mixed in the ratio 56:44 v/v (Where mobile phase A consists of potassium dihydrogen phosphate buffer of pH 3.0 and Mobile phase B consists of acetonitrile and methanol mixed in the ratio of 90:10 v/v) used at a flow rate of 0.4ml per minute. RESULTS: The retention times for losartan potassium and chlorthalidone were observed at 0.72 and 1.89 minutes. The developed method was validated as per ICH guidelines. Linearity ranges were found to be 12.5-125 μg/ml and 3.125-31.25 μg/ml for losartan potassium and chlorthalidone, respectively. CONCLUSION: This method is fast, accurate, precise and sensitive hence it can be employed for routine quality control of tablets containing both drugs in industries.
Authors: Pedro Dorado; Esther Machín; Fernando de Andrés; María-Eugenia Naranjo; Eva M Peñas-Lledó; Adrián Llerena Journal: Drug Metabol Drug Interact Date: 2012
Authors: Myriam C Salvadori; Roberto F Moreira; Bruno C Borges; Maristela H Andraus; Cristina P Azevedo; Ronilson A Moreno; Ney C Borges Journal: Clin Exp Hypertens Date: 2009-07 Impact factor: 1.749