Kai Meng1, Min Yuan2, Shujian Xu3, Lifeng Wang4, Zhe Li4, Mingyu Wang4, Rong Ma5. 1. Department of General Surgery, Qilu Hospital, Shandong University Jinan 250012, Shandong, China ; Department of Anorectal Surgery, Central Hospital of Taian Taian 271000, Shandong, China. 2. Department of Oncology, Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai 200072, China. 3. Department of Thyroid and Breast Surgery, Affiliated Hospital of Binzhou Medical College Binzhou 256603, Shandong, China. 4. Department of Anorectal Surgery, Central Hospital of Taian Taian 271000, Shandong, China. 5. Department of General Surgery, Qilu Hospital, Shandong University Jinan 250012, Shandong, China.
Abstract
OBJECTIVE: This study was to explore the expression of human cervical cancer oncogene-1 (HCCR-1) in colon cancer and its clinical significance. METHODS: RT-PCR, immunohistochemistry and Western blot assay were employed to detect HCCR-1 expression in 152 colon cancers, 43 adjacent non-cancerous tissues and 37 normal tissues. In addition, immunohistochemistry was done to detect CEA in colon cancers. RESULTS: The mRNA expression of HCCR-1 in colon cancers was higher than that in the adjacent non-cancerous tissues (P < 0.05), and the mRNA expression of HCCR-1 in adjacent non-cancerous tissues was higher than that in normal tissues (P < 0.05). The positive rate of HCCR-1 in colon cancers was 80.9%, which was higher than that in adjacent non-cancerous tissues (P < 0.05). Almost no HCCR-1 expression was observed in normal tissues, weak expression in adjacent non-cancerous tissues and strong expression in colon cancers. The positive rate of HCCR-1 in colon cancer at Duke stage B-C was 87.3%, which was higher than that in stage A colon cancer (63.6%, P < 0.05). CONCLUSION: HCCR-1 is over-expressed in colon cancers, indicating that HCCR-1 may participate in occurrence and development of colon cancer and has a correlation with the pathological progress of colon cancer progression.
OBJECTIVE: This study was to explore the expression of human cervical cancer oncogene-1 (HCCR-1) in colon cancer and its clinical significance. METHODS: RT-PCR, immunohistochemistry and Western blot assay were employed to detect HCCR-1 expression in 152 colon cancers, 43 adjacent non-cancerous tissues and 37 normal tissues. In addition, immunohistochemistry was done to detect CEA in colon cancers. RESULTS: The mRNA expression of HCCR-1 in colon cancers was higher than that in the adjacent non-cancerous tissues (P < 0.05), and the mRNA expression of HCCR-1 in adjacent non-cancerous tissues was higher than that in normal tissues (P < 0.05). The positive rate of HCCR-1 in colon cancers was 80.9%, which was higher than that in adjacent non-cancerous tissues (P < 0.05). Almost no HCCR-1 expression was observed in normal tissues, weak expression in adjacent non-cancerous tissues and strong expression in colon cancers. The positive rate of HCCR-1 in colon cancer at Duke stage B-C was 87.3%, which was higher than that in stage A colon cancer (63.6%, P < 0.05). CONCLUSION:HCCR-1 is over-expressed in colon cancers, indicating that HCCR-1 may participate in occurrence and development of colon cancer and has a correlation with the pathological progress of colon cancer progression.
Entities:
Keywords:
Colonic cancer; gene; gene expression; human cervical cancer oncogene-1
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